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- Table of Contents
Il2ra antibody (A00214-1) performs well in Flow Cytometry, IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of HEPA1-6 cells using anti-IL-2 Receptor alpha/Il2ra antibody (A00214-1).
Overlay histogram showing HEPA1-6 cells stained with A00214-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL-2 Receptor alpha/Il2ra Antibody (A00214-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA1-6 cells stained with A00214-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IL-2 Receptor alpha/Il2ra Antibody (A00214-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-IL-2 Receptor alpha/Il2ra Antibody Picoband®
Il2ra antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
Il2ra antibody was positively detected by WB in:
mouse ANA-1 whole cell, mouse NIH/3T3 whole cell, mouse RAW2647 whole cell, mouse HEPA1-6 whole cell, rat C6 whole cell, rat NRK whole cell, rat RH35 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Il2ra antibody was positively detected by IHC in:
mouse spleen tissue, rat small intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Il2ra antibody was positively detected by FC in:
HEPA1-6 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Mouse
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Product Information
Product Name
Anti-IL-2 Receptor alpha/Il2ra Antibody Picoband®
View all IL2RA Antibodies
View all IL2RA Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse IL-2 Receptor alpha/Il2ra, which shares 64.3% and 86.7% amino acid (aa) sequence identity with human and rat IL-2 Receptor alpha/Il2ra, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
30.7 kDa
Background of Il2ra
IL2RA(Interleukin 2 receptor, alpha), also called IL2R, IL2R, Alpha Chain or CD25, is a protein that in humans is encoded by the IL2RA gene.The IL2RA gene is mapped on 10p15.1. IL2RA is composed of strand-swapped "sushi-like" domains, unlike the classical cytokine receptor fold. As a result of this domain swap, IL2RA uses a composite surface to dock into a groove on IL2 that also serves as a binding site for antagonist drugs.Homodimeric alpha chains(IL2RA) result in low-affinity receptor, while homodimeric beta(IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Infection by the protozoan Trypanosoma cruzi causes Chagas disease, characterized by a reduction in the amount of IL2RA expressed on the surface of immune cells. This leads to chronic immune suppression, becoming increasingly severe over the course of many years and ultimately resulting in death if left untreated.
Cite This Product
Anti-IL-2 Receptor alpha/Il2ra Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00214-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
87%
Moderate Homology
Dog
80%
Moderate Homology
Rabbit
73%
Moderate Homology
Human
60%
Low homology
Cow
60%
Low homology
Sheep
60%
Low homology
Monkey
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Il2ra antibody
Immunohistochemistry (IHC) protocol for Il2ra antibody
Flow Cytometry (FC) protocol for Il2ra antibody
Publications
Application:
Species:
Yinqiao antipruritic formula attenuates atopic dermatitis-like skin lesions through modulating the cytokine-cytokine receptor interaction pathway
Published: 2025-04-07
Journal: FITOTERAPIA
Application: IHC
Impact Factor: 2.60
Species: Mouse
PMID: 40204047
IL-2Rα KO mice exhibit maternal microchimerism and reveal nuclear localization of IL-2Rα in lymphoid and non-lymphoid cells
Published: 2024-05-15
Journal: Frontiers in Immunology
Application: FC,WB
Impact Factor: 5.70
Species: Human,Mouse
PMID: 38812502
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A00214-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 20, 2026