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Product Info Summary
| SKU: | A00459 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-IL-21 Antibody
SKU/Catalog Number
A00459
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-21 Antibody (Catalog # A00459). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IL-21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IL-21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00459)
Host
Rabbit
Contents
IL-21 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IL-21 antibody was raised against a peptide corresponding to 15 amino acids near the center of human IL-21 precursor. The immunogen is located within the last 50 amino acids of IL-21.
Cross-reactivity
At least two isoforms of IL-21 are known to exist; this antibody will detect both isoforms. IL-21 antibody is predicted to not cross-react with other members of the interleukin protein family.
Reactive Species
A00459 is reactive to IL21 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
18.7 kDa
Background of IL21
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor gamma chain, gammac, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00459 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IHC-P: 0.5-2 μg/mL.
Antibody validated: Western Blot and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
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Western Blot Validation in Human, Mouse and Rat Tissues
Loading: 15 μg of lysates per lane.
Antibodies: IL-21 A00459, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP
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Western Blot Validation with Recombinant Protein
Loading: 30 ng of human IL-21 recombinant protein per lane.
Antibodies: IL-21 A00459 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of IL-21 in Human Tonsil
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-21 antibody (A00459) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of IL-21 in Mouse Spleen
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-21 antibody (A00459) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of IL-21 in Rat Spleen
Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-IL-21 antibody (A00459) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Induced Expression Validation of IL-21 expresssion in patients with Crohn's Disease (Monteleone et al, 2007)
Enhanced IL-21 was observed in involved but not uninvolved
CD, and was not associated with any CD phenotype, such as fibrostenosing disease. IL-21 expression was detected by anti-IL-21 antibodies (A00459).
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Regulation of IL-21 expresssion in duodenal biopsies of two patients with active Celiac disease (ACD) (Sarra et al., 2013)
(b) shows IL-21 expression levels treated with dimethyl sulfoxide (DMSO) or wortmannin (WRT).
(c) shows IL-21 expression levels with the inhibition of IL-15 antibody (aIL-15). IgG isotype was used as a control.
Both WB show IL-21 expression decreased with the treatment with WRT or the inhibition of IL-15 antibody.
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Induced Expression Validation of IL-21 expresssion in patients with Helicobacter Pylori (Hp) (Caruso et al., 2007)
(A) shows IL-21 expression levels from biopsies of patients with Hp infection and without Hp infection (n=3).
(C) shows IL-21 expression levels in CD3+LPMC cells from Hp-positive and Hp-negative patients (n=3).
Both WB show IL-21 expression increased in patients with Hp infection.
Specific Publications For Anti-IL-21 Antibody (A00459)
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