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Product Info Summary
| SKU: | M01086 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse |
| Host: | Rat |
| Application: | WB |
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Product info
Product Name
Anti- IL-5 Monoclonal Antibody
SKU/Catalog Number
M01086
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti- IL-5 Monoclonal Antibody catalog # M01086. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IL-5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01086)
Host
Rat
Contents
PBS, pH 7.0. Contains no stabilizers or preservatives
Clonality
Monoclonal
Clone Number
AFIH-9
Isotype
Rat IgG1, κ
Immunogen
Mouse partially-purified T cell clone supernatant
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
M01086 is reactive to IL5 in Mouse
Observed Molecular Weight
15 kDa
Calculated molecular weight
15.2 kDa
Background of IL5
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01086 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Western blot analysis of IL-5 using anti-IL-5 antibody (M01086).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse IL-5 Gamma protein 15ng,
Lane 2: recombinant mouse IL-5 Gamma protein 10ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rat anti-IL-5 antigen affinity purified monoclonal antibody (Catalog # M01086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Specific Publications For Anti- IL-5 Monoclonal Antibody (M01086)
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