Anti- IL-5 Monoclonal Antibody

Product Name

Anti- IL-5 Monoclonal Antibody

View all IL-5 Antibodies

SKU/Catalog Number

M01086

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti- IL-5 Monoclonal Antibody catalog # M01086. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti- IL-5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01086)

Host

Rat

Contents

PBS, pH 7.0. Contains no stabilizers or preservatives

Clonality

Monoclonal

Clone Number

AFIH-9

Isotype

Rat IgG1, κ

Immunogen

Mouse partially-purified T cell clone supernatant

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins

Reactive Species

M01086 is reactive to IL5 in Mouse

Applications

M01086 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

15.238kDa

Background of IL-5

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of IL-5 using anti-IL-5 antibody (M01086).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse IL-5 Gamma protein 15ng,
Lane 2: recombinant mouse IL-5 Gamma protein 10ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rat anti-IL-5 antigen affinity purified monoclonal antibody (Catalog # M01086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

Gene/Protein Information For IL5 (Source: Uniprot.org, NCBI)

Alternative Names

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I IL5 EDF, IL-5, TRF interleukin 5 interleukin-5|B-cell differentiation factor I|T-cell replacing factor|colony-stimulating factor, eosinophil|eosinophil differentiation factor

For more info on IL5, check out the IL5 Infographic

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In this infographic, you will see the following information for IL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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