Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A02925-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IL-9 Antibody
SKU/Catalog Number
A02925-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-9 Antibody (Catalog # A02925-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IL-9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02925-1)
Host
Rabbit
Contents
IL-9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IL-9 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IL-9. The immunogen is located within the last 50 amino acids of IL-9.
Cross-reactivity
IL-9 antibody is human specific. IL-9 antibody is predicted to no cross-react with IL-7.
Reactive Species
A02925-1 is reactive to IL9 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
15.9 kDa
Background of IL9
Interleukin 9 (IL-9) is a cytokine secreted by TH2 lymphocytes that acts as a regulator of a variety of hematopoietic cells, stimulates cell proliferation and prevents apoptosis (1,2). It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins (3). The IL-9 gene has been identified as a candidate gene for asthma (4). Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02925-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western Blot Validation in Human Spleen Tissue Lysate
Loading: 15 μg of lysates per lane.
Antibodies: IL-9, A02925-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation with Recombinant Protein
Loading: 30 ng of human IL-9 recombinant protein per lane.
Antibodies: IL-9, A02925-1 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Observed at around 22kD.
Click image to see more details
Western Blot Validation in Tissue and Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: IL-9, A02925-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Immunohistochemistry Validation of IL-9 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (A02925-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Click image to see more details
Immunohistochemistry Validation of IL-9 in Human Spleen Tissue
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-9 antibody (A02925-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-IL-9 Antibody (A02925-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IL-9 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-IL-9 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
