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Product Info Summary
| SKU: | A00651 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-IL1RN Antibody (C-term)
View all IL-1ra/IL-1F3/IL1RN Antibodies
SKU/Catalog Number
A00651
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-IL1RN Antibody (C-term) (Catalog # A00651). Tested in WB, IF, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-IL1RN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00651)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This IL1RN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-177 amino acids from the C-terminal region of human IL1RN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00651 is reactive to IL1RN in Human
Reconstitution
Calculated molecular weight
20055 Da
Background of IL-1ra/IL-1F3/IL1RN
The protein is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of its gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00651 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
FC: 1:10-1:50
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of IL1RN Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Anti-IL1RN Antibody (C-term) at 1:4000 dilution + 293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 20 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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IL1RN Antibody (C-term) (Cat. # A00651) western blot analysis in 293,Hela cell line lysates (35ug/lane). This demonstrates the IL1RN antibody detected the IL1RN protein (arrow).
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IL1RN Antibody (C-term) (Cat. #A00651) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For IL1RN (Source: Uniprot.org, NCBI)
Gene Name
IL1RN
Full Name
Interleukin-1 receptor antagonist protein
Weight
20055 Da
Alternative Names
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, Anakinra, IL1RN, IL1F3, IL1RA IL1RN DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4 interleukin 1 receptor antagonist interleukin-1 receptor antagonist protein|IL1 inhibitor|intracellular IL-1 receptor antagonist type II|intracellular interleukin-1 receptor antagonist (icIL-1ra)|type II interleukin-1 receptor antagonist
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1RN, check out the IL1RN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1RN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL1RN Antibody (C-term) (A00651)
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