Click image to see more details
Product Info Summary
| SKU: | A00651 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-IL1RN Antibody (C-term)
SKU/Catalog Number
A00651
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-IL1RN Antibody (C-term) (Catalog # A00651). Tested in WB, IF, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-IL1RN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00651)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This IL1RN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-177 amino acids from the C-terminal region of human IL1RN.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00651 is reactive to IL1RN in Human
Calculated molecular weight
20.1 kDa
Background of IL1RN
The protein is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of its gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00651 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
FC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Confocal immunofluorescent analysis of IL1RN Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Click image to see more details
Anti-IL1RN Antibody (C-term) at 1:4000 dilution + 293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 20 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
IL1RN Antibody (C-term) (Cat. # A00651) western blot analysis in 293,Hela cell line lysates (35ug/lane). This demonstrates the IL1RN antibody detected the IL1RN protein (arrow).
Click image to see more details
IL1RN Antibody (C-term) (Cat. #A00651) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-IL1RN Antibody (C-term) (A00651)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-IL1RN Antibody (C-term)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-IL1RN Antibody (C-term)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
