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Product Info Summary
| SKU: | A00963-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL22 Antibody Picoband®
SKU/Catalog Number
A00963-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL22 Antibody Picoband® catalog # A00963-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL22 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00963-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL22 recombinant protein (Position: L34-V179).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00963-3 is reactive to Il22 in Mouse, Rat
Observed Molecular Weight
20 kDa
Calculated molecular weight
20.1 kDa
Background of Il22
Interleukin-22 (IL-22), also known as ILTIF, is protein that in humans is encoded by the IL22 gene. IL-22 a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. Using FISH, the IL22 gene is mapped to chromosome 12q15, close to the IFNG and the herpesvirus saimiri-induced AK155 genes. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. It also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. In some contexts, the pro-inflammatory versus tissue-protective functions of IL-22 are regulated by the often co-expressed cytokine IL-17A.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00963-3 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse kidney tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of IL22 using anti-IL22 antibody (A00963-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse kidney tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL22 antigen affinity purified polyclonal antibody (Catalog # A00963-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL22 at approximately 20KD. The expected band size for IL22 is at 20KD.
Specific Publications For Anti-IL22 Antibody Picoband® (A00963-3)
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Customer Q&As
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16 Customer Q&As for Anti-IL22 Antibody Picoband®
Question
We ordered your anti-IL22 antibody for WB on zone of skin a few years ago. I am using mouse, and We want to use the antibody for ELISA next. We need examining zone of skin as well as blood in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
I viewed the website and datasheets of our anti-IL22 antibody and it seems that A00963-3 has been validated on mouse in both WB and ELISA. Thus A00963-3 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-03
Question
We have been able to see staining in rat blood. Any tips? Is anti-IL22 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
Based on literature blood does express IL22. Based on Uniprot.org, IL22 is expressed in zone of skin, blood, among other tissues. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-14
Question
My question regards using your anti-IL22 antibody for regulation of tyrosine phosphorylation of stat protein studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
We appreciate your inquiry. This A00963-3 anti-IL22 antibody is tested on mouse kidney tissue, tissue lysate, brain tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-07
Question
Is there a BSA free version of anti-IL22 antibody A00963-3 available?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL22 antibody A00963-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-19
Question
My question regarding product A00963-3, anti-IL22 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00963-3 anti-IL22 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-06
Question
See below the WB image, lot number and protocol we used for blood using anti-IL22 antibody A00963-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
Would anti-IL22 antibody A00963-3 work for ELISA with blood?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to the expression profile of blood, IL22 is highly expressed in blood. So, it is likely that anti-IL22 antibody A00963-3 will work for ELISA with blood.
Boster Scientific Support
Answered: 2019-08-30
Question
Our lab were well pleased with the WB result of your anti-IL22 antibody. However we have observed positive staining in blood secreted. using this antibody. Is that expected? Could you tell me where is IL22 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to literature, blood does express IL22. Generally IL22 expresses in secreted. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-30
Question
We are currently using anti-IL22 antibody A00963-3 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The anti-IL22 antibody (A00963-3) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-28
Question
I see that the anti-IL22 antibody A00963-3 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-22
Question
I am interested in to test anti-IL22 antibody A00963-3 on mouse blood for research purposes, then I may be interested in using anti-IL22 antibody A00963-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
The products we sell, including anti-IL22 antibody A00963-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-15
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-IL22 antibody A00963-3. Let me know if you need anything else.
S. Walker
Verified customer
Asked: 2018-08-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-17
Question
Is a blocking peptide available for product anti-IL22 antibody (A00963-3)?
Verified Customer
Verified customer
Asked: 2018-04-24
Answer
We do provide the blocking peptide for product anti-IL22 antibody (A00963-3). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-04-24
Question
Is this A00963-3 anti-IL22 antibody reactive to the isotypes of IL22?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
The immunogen of A00963-3 anti-IL22 antibody is E. coli-derived mouse IL22 recombinant protein (Position: L34-V179). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-05
Question
Would A00963-3 anti-IL22 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Lewis
Verified customer
Asked: 2017-04-11
Answer
It shows on the product datasheet, A00963-3 anti-IL22 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-04-11
Question
I was wanting to use your anti-IL22 antibody for ELISA for mouse blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse blood identification?
G. Brown
Verified customer
Asked: 2015-11-13
Answer
You can see on the product datasheet, A00963-3 anti-IL22 antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-11-13
