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Product Info Summary
| SKU: | PB9761 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL2 Receptor beta/IL2RB Antibody Picoband®
SKU/Catalog Number
PB9761
PB0812 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL2 Receptor beta/IL2RB Antibody Picoband® catalog # PB9761. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL2 Receptor beta/IL2RB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9761)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL2RB, different from the related human sequence by sixteen amino acids, and from the related rat sequence by fourteen amino acids.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9761 is reactive to Il2rb in Mouse
Observed Molecular Weight
75 kDa
Calculated molecular weight
60.5 kDa
Background of Il2rb
IL2RB (INTERLEUKIN 2 RECEPTOR, BETA), also called CD122, is a protein that in humans is encoded by the IL2RB gene. The gene represents the beta subunit and is a type I membrane protein. IL2RB is mapped to 22q12.3. The IL2RB gene contains 10 exons spanning about 24.3 kb and that the gene product is encoded by exons 2-10. The IL2 receptor is present in 3 forms with respect to binding ability to IL2: high, intermediate, and low affinity forms. Analysis of mutants of the beta chain of the IL2 receptor reveals that the granulocyte and monocyte differentiation signals are triggered by different cytoplasmic domains, showing that the signaling pathways responsible for these unique developmental outcomes are separable.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9761 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: Mouse Lung Tissue, Mouse Spleen Tissue, Mouse Testis Tissue
Validation Images & Assay Conditions
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Western blot analysis of IL2RB using anti-IL2RB antibody (PB9761).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.
Lane 1: Mouse Lung Tissue Lysate,
Lane 2: Mouse Spleen Tissue Lysate,
Lane 3: Mouse Testis Tissue Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RB antigen affinity purified polyclonal antibody (Catalog # PB9761) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2RB at approximately 75 kDa. The expected band size for IL2RB is at 75 kDa.
Specific Publications For Anti-IL2 Receptor beta/IL2RB Antibody Picoband® (PB9761)
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1 Customer Q&As for Anti-IL2 Receptor beta/IL2RB Antibody Picoband®
Question
We are currently using anti-IL2 Receptor beta/IL2RB antibody PB9761 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The anti-IL2 Receptor beta/IL2RB antibody (PB9761) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-23
