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Anti- IL3 Monoclonal Antibody
Boster Bio Anti- IL3 Monoclonal Antibody catalog # M02435. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti- IL3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02435)
PBS, pH 7.0. Contains no stabilizers or preservatives
COS-expressed, recombinant mouse IL-3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M02435 is reactive to Il3 in Mouse
M02435 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL3 using anti-IL3 antibody (M02435).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse IL3 protein 15ng,
Lane 2: recombinant mouse IL3 protein 10ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rat anti-IL3 antigen affinity purified monoclonal antibody (Catalog # M02435) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rat IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Gene/Protein Information For Il3 (Source: Uniprot.Org, NCBI)
Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor Il3|B, BPA, Csfm, Csfmu, HCGF, Il, Il-3, MCGF, P, PSF|interleukin 3|interleukin-3|P-cell-stimulating factor|hematopoietic growth factor|mast cell growth factor|multipotential colony-stimulating factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il3, check out the Il3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for M02435
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