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Product Info Summary
| SKU: | A09802-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB, ELISA (Cap) |
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Product info
Product Name
Anti-IL36 alpha/IL36A Antibody Picoband®
SKU/Catalog Number
A09802-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL36 alpha/IL36A Antibody Picoband® catalog # A09802-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL36 alpha/IL36A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09802-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL36 alpha recombinant protein (Position: K6-D151).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09802-3 is reactive to IL36A in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
17.7 kDa
Background of IL36A
Interleukin-36 alpha also known as interleukin-1 family member 6 (IL1F6) is a protein that in humans is encoded by the IL36A gene. The protein encoded by this gene is a cytokine that can activate NF-kappa-B and MAPK signaling pathways to generate an inflammatory response. The encoded protein functions primarily in skin and demonstrates increased expression in psoriasis. In addition, decreased expression of this gene has been linked to a poor prognosis in both hepatocellular carcinoma and colorectal cancer patients.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09802-3 is guaranteed for IHC, WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue, human Hela whole cell, human placenta tissue, human MCF-7 whole cell, human HepG2 whole cell
IHC: human intestinal cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of IL36 alpha using anti-IL36 alpha antibody (A09802-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: human Hela whole cell lysates,
Lane 6: human placenta tissue lysates,
Lane 7: human MCF-7 whole cell lysates,
Lane 8: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL36 alpha antigen affinity purified polyclonal antibody (Catalog # A09802-3) at 0.5 μg/mL overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL36 alpha at approximately 25KD. The expected band size for IL36 alpha is at 17KD.
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IHC analysis of IL36 alpha using anti-IL36 alpha antibody (A09802-3).
IL36 alpha was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-IL36 alpha Antibody (A09802-3) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-IL36 alpha/IL36A Antibody Picoband® (A09802-3)
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1 Customer Q&As for Anti-IL36 alpha/IL36A Antibody Picoband®
Question
We are currently using anti-IL36 alpha/IL36A antibody A09802-3 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
C. Anderson
Verified customer
Asked: 2013-11-04
Answer
The anti-IL36 alpha/IL36A antibody (A09802-3) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-04
