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Product Info Summary
| SKU: | M00230 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-IL4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00230
BM5023 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IL4 Rabbit Monoclonal Antibody catalog # M00230. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00230)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AADD-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IL4
Reactive Species
M00230 is reactive to IL4 in Human
Observed Molecular Weight
15 kDa
Calculated molecular weight
17.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00230 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Positive Control
WB: human HEL whole cell
Validation Images & Assay Conditions
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Western blot analysis of IL-4 using anti-IL-4 antibody (M00230).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-4 antigen affinity purified monoclonal antibody (M00230) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL-4 at approximately 15 kDa. The expected band size for IL-4 is at 17 kDa.
Specific Publications For Anti-IL4 Rabbit Monoclonal Antibody (M00230)
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Customer Q&As
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15 Customer Q&As for Anti-IL4 Rabbit Monoclonal Antibody
Question
I would like to test anti-IL4 Rabbit Monoclonal antibody M00230 on human oocyte for research purposes, then I may be interested in using anti-IL4 Rabbit Monoclonal antibody M00230 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The products we sell, including anti-IL4 Rabbit Monoclonal antibody M00230, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-28
Question
Would M00230 anti-IL4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
You can see on the product datasheet, M00230 anti-IL4 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-13
Question
I see that the anti-IL4 Rabbit Monoclonal antibody M00230 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-11-14
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for oocyte using anti-IL4 Rabbit Monoclonal antibody M00230. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-03
Question
Is this M00230 anti-IL4 Rabbit Monoclonal antibody reactive to the isotypes of IL4?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
The immunogen of M00230 anti-IL4 Rabbit Monoclonal antibody is A synthesized peptide derived from human IL4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-14
Question
Is a blocking peptide available for product anti-IL4 Rabbit Monoclonal antibody (M00230)?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We do provide the blocking peptide for product anti-IL4 Rabbit Monoclonal antibody (M00230). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-11
Question
We are currently using anti-IL4 Rabbit Monoclonal antibody M00230 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The anti-IL4 Rabbit Monoclonal antibody (M00230) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-31
Question
Can you help my question with product M00230, anti-IL4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00230 anti-IL4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-30
Question
I have attached the WB image, lot number and protocol we used for oocyte using anti-IL4 Rabbit Monoclonal antibody M00230. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-22
Question
We ordered your anti-IL4 Rabbit Monoclonal antibody for WB on blood in the past. I am using human, and We want to use the antibody for IF next. I am interested in examining blood as well as oocyte in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-09-14
Answer
I viewed the website and datasheets of our anti-IL4 Rabbit Monoclonal antibody and it seems that M00230 has been validated on human in both WB and IF. Thus M00230 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-14
Question
Our lab were well pleased with the WB result of your anti-IL4 Rabbit Monoclonal antibody. However we have observed positive staining in oocyte secreted. using this antibody. Is that expected? Could you tell me where is IL4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-08-17
Answer
From literature, oocyte does express IL4. Generally IL4 expresses in secreted. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-08-17
Question
I was wanting to use your anti-IL4 Rabbit Monoclonal antibody for WB for human oocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human oocyte identification?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
As indicated on the product datasheet, M00230 anti-IL4 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human oocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-14
Question
We have been able to see staining in human oocyte. Are there any suggestions? Is anti-IL4 Rabbit Monoclonal antibody supposed to stain oocyte positively?
G. Lewis
Verified customer
Asked: 2017-05-23
Answer
From what I have seen in literature oocyte does express IL4. From what I have seen in Uniprot.org, IL4 is expressed in oocyte, blood, among other tissues. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-05-23
Question
Will anti-IL4 Rabbit Monoclonal antibody M00230 work for WB with oocyte?
R. Baker
Verified customer
Asked: 2015-11-10
Answer
According to the expression profile of oocyte, IL4 is highly expressed in oocyte. So, it is likely that anti-IL4 Rabbit Monoclonal antibody M00230 will work for WB with oocyte.
Boster Scientific Support
Answered: 2015-11-10
Question
Do you have a BSA free version of anti-IL4 Rabbit Monoclonal antibody M00230 available?
S. Parker
Verified customer
Asked: 2015-01-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL4 Rabbit Monoclonal antibody M00230 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-01-08
