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Product Info Summary
| SKU: | PA1619 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL5RA Antibody
View all IL-5R alpha/CD125 Antibodies
SKU/Catalog Number
PA1619
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL5RA Antibody catalog # PA1619. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL5RA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1619)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IL5RA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1619 is reactive to IL5RA in Human
Applications
PA1619 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
47.685kDa
Background of IL-5R alpha/CD125
IL5RA (INTERLEUKIN 5 RECEPTOR, ALPHA), also known as CD125, is a subunit of the Interleukin-5 receptor. IL5RA also denotes its human gene. The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor which is composed of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The IL5RA gene is mapped to 3p26-p24 by Southern blot analysis of DNA from a panel of mouse-human hybrid somatic cell lines complemented by in situ hybridization. And its Cytogenetic location is 3p26.2. Within this 15-residue stretch of IL5RA, the C-terminal phenylalanine is critical. The binding of this protein to IL5 depends on the beta subunit which is activated by the ligand binding and is required for the biological activities of IL5.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-IL5RA antibody, PA1619, Western blotting
All lanes: Anti IL5RA (PA1619) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: 293T Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: JURKAT Whole Cell Lysate at 40ug
Lane 8: RAJI Whole Cell Lysate at 40ug
Lane 9: CEM Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 60KD
Protein Target Info & Infographic
Gene/Protein Information For IL5RA (Source: Uniprot.org, NCBI)
Gene Name
IL5RA
Full Name
Interleukin-5 receptor subunit alpha
Weight
47.685kDa
Superfamily
type I cytokine receptor family
Alternative Names
CD125 antigen; CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5 receptor subunit alpha; IL5R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; IL-5R-alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; interleukin-5 receptor subunit alpha; MGC26560 IL5RA CD125, CDw125, HSIL5R3, IL5R interleukin 5 receptor subunit alpha interleukin-5 receptor subunit alpha|CD125 antigen|IL-5 receptor subunit alpha|IL-5R subunit alpha|interleukin 5 receptor type 3|interleukin 5 receptor, alpha|interleukin-5 receptor alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL5RA, check out the IL5RA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL5RA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL5RA Antibody (PA1619)
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No publications found for PA1619
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-IL5RA Antibody
Question
Would PA1619 anti-IL5RA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
F. Edwards
Verified customer
Asked: 2019-12-05
Answer
It shows on the product datasheet, PA1619 anti-IL5RA antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-05
Question
We are currently using anti-IL5RA antibody PA1619 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The anti-IL5RA antibody (PA1619) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-06
Question
Is a blocking peptide available for product anti-IL5RA antibody (PA1619)?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
We do provide the blocking peptide for product anti-IL5RA antibody (PA1619). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-30
Question
I see that the anti-IL5RA antibody PA1619 works with WB, what is the protocol used to produce the result images on the product page?
A. Miller
Verified customer
Asked: 2013-04-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-04-08
