Product Info Summary
SKU: | A04443-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-ILF2 Antibody
SKU/Catalog Number
A04443-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ILF2 Antibody catalog # A04443-1. Tested in WB,IP,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ILF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04443-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human ZNF148.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04443-1 is reactive to ILF2 in Human, Mouse, Rat
Applications
A04443-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43.062kDa
Background of ILF2
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Boopathi E., J. Biol. Chem. 279:35242-35254(2004).
Beausoleil S.A., Nat. Biotechnol. 24:1285-1292(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
IHC: 1:50-1:200
FC: 1:50-1:100
IP: 1:10-1:20
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of ILF2 using Anti-ILF2 Antibody (A04443-1).
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ILF2 antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-ILF2 Antibody A04443-1
Western blot analysis of ILF2 on different cell lysates using anti-ILF2 antibody at 1/500 dilution.Positive control:
Lane 1: Mouse testis tissue
Lane 2: HL-60
Lane 3: SH-SY-5Y
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ILF2 (Source: Uniprot.org, NCBI)
Gene Name
ILF2
Full Name
Interleukin enhancer-binding factor 2
Weight
43.062kDa
Alternative Names
interleukin enhancer binding factor 2, 45kD; interleukin enhancer binding factor 2, 45kDa; interleukin enhancer-binding factor 2; MGC8391; NF45PRO3063; Nuclear factor of activated T-cells 45 kDa; nuclear factor of activated T-cells, 45-kDa ILF2 NF45, PRO3063 interleukin enhancer binding factor 2 interleukin enhancer-binding factor 2|interleukin enhancer binding factor 2, 45kD|interleukin enhancer binding factor 2, 45kDa|nuclear factor of activated T-cells, 45-kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ILF2, check out the ILF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ILF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ILF2 Antibody (A04443-1)
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No publications found for A04443-1
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Customer Reviews
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