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Product Info Summary
| SKU: | A09422 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-INSL4 Antibody
SKU/Catalog Number
A09422
Size
100μl
Form
Liquid
Description
Boster Bio Anti-INSL4 Antibody catalog # A09422. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-INSL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09422)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human INSL4. AA range:41-90
Reactive Species
A09422 is reactive to INSL4 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
15.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09422 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549 cells, using INSL4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using INSL4 Polyclonal Antibody
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Western blot analysis of lysates from HT-29 cells, using INSL4 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-INSL4 Antibody (A09422)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-INSL4 Antibody
Question
I was wanting to use your anti-INSL4 antibody for WB for human chorionic villus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human chorionic villus identification?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
You can see on the product datasheet, A09422 anti-INSL4 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human chorionic villus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-26
Question
We are currently using anti-INSL4 antibody A09422 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
The anti-INSL4 antibody (A09422) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-25
Question
Do you have a BSA free version of anti-INSL4 antibody A09422 available?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-INSL4 antibody A09422 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-05
Question
Is this A09422 anti-INSL4 antibody reactive to the isotypes of INSL4?
S. Baker
Verified customer
Asked: 2019-08-16
Answer
The immunogen of A09422 anti-INSL4 antibody is Synthesized peptide derived from the Internal region of human INSL4. at AA range: 10-90. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-16
Question
My question regarding product A09422, anti-INSL4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Carter
Verified customer
Asked: 2019-02-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09422 anti-INSL4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-26
Question
Does A09422 anti-INSL4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-04
Answer
As indicated on the product datasheet, A09422 anti-INSL4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-04
Question
I see that the anti-INSL4 antibody A09422 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-06-07
