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Product Info Summary
| SKU: | M00067-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC |
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Product info
Product Name
Anti-Insulin Rabbit Monoclonal Antibody
SKU/Catalog Number
M00067-1
BM4310 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Insulin Rabbit Monoclonal Antibody catalog # M00067-1. Tested in IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00067-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DEA-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Insulin
Reactive Species
M00067-1 is reactive to INS in Human, Mouse, Rat
Observed Molecular Weight
12 kDa
Calculated molecular weight
12.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00067-1 is guaranteed for IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
IHC: mouse pancreas tissue
ICC/IF: Beta-TC-6 cell
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded mouse pancreas, using Insulin Antibody.
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IF analysis of Insulin using anti-Insulin antibody (M00067-1).
Insulin was detected in an immunocytochemical section of Beta-TC-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 rabbit anti-Insulin Antibody (M00067-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Insulin Rabbit Monoclonal Antibody (M00067-1)
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Customer Q&As
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2 Customer Q&As for Anti-Insulin Rabbit Monoclonal Antibody
Question
Can we use basic antigen retrieval with M00067-1 antibody, as alternatives for citrate buffer?
Verified customer
Asked: 2019-10-24
Answer
For the Anti-Insulin Rabbit Monoclonal Antibody M00067-1, it is suggested to use Tris-EDTA buffer for the antigen retrieval buffer with the compositions; 10 mM Tris(hydroxymethyl)methyl aminomethane THAM, 1 mM EDTA, 0.05% Tween 20, pH 9.0.
Boster Scientific Support
Answered: 2019-10-29
Question
Would you please check the IHC image for this mAb: https://www.bosterbio.com/anti-insulin-rabbit-monoclonal-antibody-m00067-1-boster.html?options=cart I
Verified Customer
Verified customer
Asked: 2014-06-11
Answer
We have updated the image. https://www.bosterbio.com/anti-insulin-rabbit-monoclonal-antibody-m00067-1-boster.html?options=cart
Boster Scientific Support
Answered: 2014-06-11
