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Product Info Summary
| SKU: | PA1620 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Insulin Receptor/INSR Antibody
SKU/Catalog Number
PA1620
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Insulin Receptor/INSR Antibody catalog # PA1620. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Insulin Receptor/INSR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1620)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Insulin Receptor, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1620 is reactive to INSR in Human, Mouse, Rat
Applications
PA1620 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
155 kDa
Calculated molecular weight
156.333kDa
Background of INSR
INSR (INSULIN RECEPTOR) is a tetramer of 2 alpha and 2 beta subunits that are coded by a single gene and are joined by disulfide bonds, a mechanism parallel to that of its ligand, insulin. It belongs to the large class of tyrosine kinase receptors. The insulin receptor gene is mapped to 19p13.2. The insulin receptor mediates their activity by causing the addition of a phosphate group to particular tyrosines on certain proteins within a cell. The INSR gene spans more than 120 kb and has 22 exons. Functional studies of the INSR SNPs show no effect on mRNA levels or splicing in peripheral blood leukocytes or on binding of insulin to mononuclear cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-Insulin Receptor antibody, PA1620, Western blotting
All lanes: Anti Insulin Receptor(PA1620) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: PANC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 155KD
Observed bind size: 155KD
Protein Target Info & Infographic
Gene/Protein Information For INSR (Source: Uniprot.org, NCBI)
Gene Name
INSR
Full Name
Insulin receptor
Weight
156.333kDa
Superfamily
protein kinase superfamily
Alternative Names
CD220 antigen; CD220; EC 2.7.10; EC 2.7.10.1; HHF5; insulin receptor; IR INSR CD220, HHF5 insulin receptor insulin receptor|IR
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on INSR, check out the INSR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for INSR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Insulin Receptor/INSR Antibody (PA1620)
Hello CJ!
PA1620 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births
Indomethacin decreases insulin secretion by reducing KCa3.1 as a biomarker of pancreatic tumor and causes apoptotic cell death
Wang Q, Sun X, Li X, Dong X, Li P, Zhao L. Mol Med Rep. 2015 Jan;11(1):151-8. Doi: 10.3892/Mmr.2014.2762. Epub 2014 Oct 23. Resveratrol Attenuates Intermittent Hypoxia-Induced Insulin Resistance In Rats: Involvement Of Sirtuin 1 And The Phosphatid...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Insulin Receptor/INSR Antibody
Question
Is this PA1620 anti-Insulin Receptor/INSR antibody reactive to the isotypes of INSR?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The immunogen of PA1620 anti-Insulin Receptor/INSR antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Insulin Receptor(1353-1372aa RSYEEHIPYTHMNGGKKNGR), different from the related rat and mouse sequences by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-21
Question
I was wanting to use your anti-Insulin Receptor/INSR antibody for WB for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
As indicated on the product datasheet, PA1620 anti-Insulin Receptor/INSR antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-10
Question
Will anti-Insulin Receptor/INSR antibody PA1620 work for WB with placenta?
J. Jones
Verified customer
Asked: 2015-09-25
Answer
According to the expression profile of placenta, INSR is highly expressed in placenta. So, it is likely that anti-Insulin Receptor/INSR antibody PA1620 will work for WB with placenta.
Boster Scientific Support
Answered: 2015-09-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Insulin Receptor/INSR antibody PA1620. Let me know if you need anything else.
W. Lewis
Verified customer
Asked: 2015-08-19
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-08-19
