Anti-Insulin Receptor/INSR Antibody
Rabbit IgG polyclonal antibody for Insulin receptor(INSR) detection. Tested with WB in Human;Mouse;Rat. Cited in 1 publication(s).
Anti-Insulin Receptor/INSR Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-Insulin Receptor/INSR Antibody
See all INSR primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for INSULIN RECEPTOR/INSR detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. INSULIN RECEPTOR/INSR information: Molecular Weight: 156333 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.|
|Cite This Product||Anti-Insulin Receptor/INSR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1620)|
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|Anti-Insulin Receptor/INSR Antibody may replace the following items: sc 80647|sc 14374|sc 23019|sc 23020|sc 367003|sc 393552|sc 14378|sc 14373|sc 136435|sc 514852|sc 514853|sc 136435 X.|
|Reactivity/Species||Human, Mouse, Rat|
|Predicted Reactivity||Canine, Horse, Zebrafish
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Insulin Receptor(1353-1372aa RSYEEHIPYTHMNGGKKNGR), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For INSR (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3643|
|Species Of This Entry||Human|
|Protein Name||Insulin receptor|
|Superfamily||protein kinase superfamily|
|Alternative Names||INSR|CD220 antigen; CD220; EC 2.7.10; EC 126.96.36.199; HHF5; insulin receptor; IR|
|Post Tranlational Modifications||Phosphorylation; Methylation; Oxidation; Carboxylation; Cleavage; Glycosylation; Decarboxylation; Acetylation; Dephosphorylation; Acylation|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. Late endosome. Lysosome. Binding of insulin to INSR induces internalization and lysosomal degradation of the receptor, a means for downregulating this signaling pathway after stimulation. In the presence of SORL1, internalized INSR molecules are redirected back to the cell surface, thereby preventing their lysosomal catabolism and strengthening insulin signal reception.|
|Gene Location||19p13.2, on Chromasome 19, gene sequence: NC_000019.10 (7112257..7294414, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For INSR (Source: Uniprot.org, NCBI)
|Protein Function||Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src- homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti- apoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K- AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.|
|Tissue Specificity||Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.|
|Interacting Proteins||AGAP2; ARRB2; DUSP18; ENPP1; GRB10; IGF1; INS; IRS1; Irs1; Irs2; KRT31; PIK3R1; PLCG1; PTPN1; PTPN11; PTPRR; SH2B1; SHC1; Trim72|
|Related Diseases||Insulin Resistance; Diabetes Mellitus; Diabetes Mellitus, Non-insulin-dependent; Malignant Neoplasms; Obesity; Neoplasms; Hyperinsulinism; Polycystic Ovary Syndrome; Insulin Sensitivity; Hypertensive Disease; Hyperglycemia; Malignant Neoplasm Of Breast; Mammary Neoplasms; Diabetes Mellitus, Experimental; Essential Hypertension; Donohue Syndrome; Atherosclerosis; Cell Transformation, Neoplastic; Metabolic Syndrome X; Metabolic Diseases|
|Related Pathways||Secretion; Pathogenesis; Insulin Secretion; Cell Proliferation; Transport; Glucose Homeostasis; Aging; Cell Growth; Localization; Lactation; Dna Methylation; Cell Cycle; Methylation; Cell Differentiation; Glycolysis; Gene Silencing; Reverse Transcription; Angiogenesis; Rna Interference; Mating|
|Background||INSR(INSULIN RECEPTOR) is a tetramer of 2 alpha and 2 beta subunits that are coded by a single gene and are joined by disulfide bonds, a mechanism parallel to that of its ligand, insulin. It belongs to the large class of tyrosine kinase receptors. The insulin receptor gene is mapped to 19p13.2. The insulin receptor mediates their activity by causing the addition of a phosphate group to particular tyrosines on certain proteins within a cell. The INSR gene spans more than 120 kb and has 22 exons. Functional studies of the INSR SNPs show no effect on mRNA levels or splicing in peripheral blood leukocytes or on binding of insulin to mononuclear cells.|
|Scientific References||PMID: 2859121 by Ebina Y., et al. The human insulin receptor cDNA: the structural basis for hormone- activated transmembrane signalling.|
PMID: 2983222 by Ullrich A., et al. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.
Our Boster Quality Guarantee for Anti-Insulin Receptor/INSR Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti Insulin Receptor(PA1620) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: PANC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 155KD
Observed bind size: 155KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 1
|Wang Q, Sun X, Li X, Dong X, Li P, Zhao L. Mol Med Rep. 2015 Jan;11(1):151-8. Doi: 10.3892/Mmr.2014.2762. Epub 2014 Oct 23. Resveratrol Attenuates Intermittent Hypoxia-Induced Insulin Resistance In Rats: Involvement Of Sirtuin 1 And The Phosphatid...||PubMed ID 25352008|
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