Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®

Product Name

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband®

View all PDK1 Antibodies

SKU/Catalog Number

PA1993
BA4499 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody catalog # PA1993. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1993)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1993 is reactive to PDK1 in Human, Rat

Observed Molecular Weight

85-91 kDa

Calculated molecular weight

49.2 kDa

Background of PDK1

PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1993 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Western blot, 0.1-0.5μg/ml, Human, Rat

Positive Control

WB: Rat Heart Tissue, Rat Skeletal Muscle Tissue, HELA Cell, M231 Cell, COLO320 Cell, SW620 Cell
IHC: Human Prostatic Cancer Tissue

Validation Images & Assay Conditions

See full target information PDK1

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