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- Table of Contents
INSR antibody (A00447-2) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Western blot analysis of Insulin Receptor/INSR using anti-Insulin Receptor/INSR antibody (A00447-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat pancreas tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Insulin Receptor/INSR antigen affinity purified polyclonal antibody (Catalog # A00447-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Insulin Receptor/INSR at approximately 156 kDa. The expected band size for Insulin Receptor/INSR is at 156 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat pancreas tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Insulin Receptor/INSR antigen affinity purified polyclonal antibody (Catalog # A00447-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Insulin Receptor/INSR at approximately 156 kDa. The expected band size for Insulin Receptor/INSR is at 156 kDa.
Designing a Western blot for INSR? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the INSR Western Blot GuideBundle Save
Anti-Insulin Receptor/INSR Antibody Picoband®
INSR antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, WB |
Tested Applications
INSR antibody was positively detected by WB in:
rat liver tissue, rat pancreas tissue, mouse liver tissue, mouse pancreas tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Recommend
Dog
Predicted
Predicted
Bovine
Predicted
Predicted
Rabbit
Predicted
Predicted
Sheep
Predicted
Predicted
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-Insulin Receptor/INSR Antibody Picoband®
View all INSR Antibodies
View all INSR Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Insulin Receptor/INSR recombinant protein (Position: E168-N621).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
156 kDa
Calculated Molecular Weight
156.3 kDa
Background of INSR
INSR(INSULIN RECEPTOR) is a tetramer of 2 alpha and 2 beta subunits that are coded by a single gene and are joined by disulfide bonds, a mechanism parallel to that of its ligand, insulin. It belongs to the large class of tyrosine kinase receptors. The insulin receptor gene is mapped to 19p13.2. The insulin receptor mediates their activity by causing the addition of a phosphate group to particular tyrosines on certain proteins within a cell. The INSR gene spans more than 120 kb and has 22 exons. Functional studies of the INSR SNPs show no effect on mRNA levels or splicing in peripheral blood leukocytes or on binding of insulin to mononuclear cells.
Cite This Product
Anti-Insulin Receptor/INSR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00447-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
98%
High Homology
Cow
98%
High Homology
Rabbit
98%
High Homology
Sheep
98%
High Homology
Mouse
96%
High Homology
Rat
96%
High Homology
Monkey
94%
High Homology
Zebrafish
68%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for INSR antibody
Publications
Application:
Species:
Identification of E2F8 as a Transcriptional Regulator of Gluconeogenesis in Primary Mouse Hepatocytes
Published: 2019-12-18
Journal: BIOCHEMISTRY-MOSCOW
Application: WB
Impact Factor: 1.89
Species: Mouse
PMID: 31870257
Resveratrol attenuates intermittent hypoxia‑induced insulin resistance in rats: Involvement of Sirtuin 1 and the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase/AKT pathway
Published: 2014-10-23
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.48
Species: Rat
PMID: 25352008
Insulin suppresses the expression and function of breast cancer resistance protein in primary cultures of rat brain microvessel endothelial cells
Published: 2011-12-30
Journal: Pharmacological Reports
Application: WB
Impact Factor: 2.50
Species: Rat
PMID: 21602604
Insulin regulates P-glycoprotein in rat brain microvessel endothelial cells via an insulin receptor-mediated PKC/NF-κB pathway but not a PI3K/Akt pathway
Published: 2008-11-21
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 2.38
Species: Rat
PMID: 19049803
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Product Promise
We promise Anti-Insulin Receptor/INSR Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00447-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 19, 2026