Product Info Summary
SKU: | P00306 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) Antibody
View all IFN-alpha/beta R1 Antibodies
SKU/Catalog Number
P00306
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535, 539) Antibody (Catalog # P00306). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00306)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 of human interferon-α, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00306 is reactive to IFNAR1 in Human
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
63525 MW
Background of IFN-alpha/beta R1
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-α Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine-535 and Ser-539 in the IFNAR1 (Kumar et al., 2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00306 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of immunoprecipitates from HEK 293 cells transfected with 1) Mock, 2) IFNAR1 WT, and 3) IFNAR1 S535A and S539A mutants. Specific immunolabeling of the ~110 kDa to ~130 kDa IFNAR1 WT (2) is shown in the first blot, as the immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (3). The specific immunolabeling is blocked by the phosphopeptide (+) used as the antigen in the second blot.
Protein Target Info & Infographic
Gene/Protein Information For IFNAR1 (Source: Uniprot.org, NCBI)
Gene Name
IFNAR1
Full Name
Interferon alpha/beta receptor 1
Weight
63525 MW
Superfamily
type II cytokine receptor family
Alternative Names
Alpha-Type Antiviral Protein, AVP, Beta-Type Antiviral Protein, CRF2-1, Cytokine Receptor Class-II Member 1, Cytokine Receptor Family 2 Member 1, IFN-Alpha/Beta Receptor 1, IFNBR, IFN-Alpha-REC, IFNAR, IFN-R-1, IFRC, Interferon Alpha/Beta Receptor 1, Interferon-Alpha/Beta Receptor Alpha Chain, Interferon Alpha And Beta Receptor Subunit 1, Interferon (Alpha, Beta And Omega) Receptor 1, Interferon-Beta Receptor 1, Interferon Receptor 1, Type I Interferon Receptor 1 IFNAR1 AVP, IFN-alpha-REC, IFNAR, IFNBR, IFRC interferon alpha and beta receptor subunit 1 interferon alpha/beta receptor 1|CRF2-1|IFN-R-1|IFN-alpha/beta receptor 1|IFNalpha/beta receptor 1|alpha-type antiviral protein|beta-type antiviral protein|cytokine receptor class-II member 1|cytokine receptor family 2 member 1|interferon (alpha, beta and omega) receptor 1|interferon receptor 1|interferon-alpha/beta receptor alpha chain|interferon-beta receptor 1|type I interferon receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFNAR1, check out the IFNAR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFNAR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) Antibody (P00306)
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No publications found for P00306
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Customer Q&As
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16 Customer Q&As for Anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) Antibody
Question
Does anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
According to the expression profile of leukocyte, IFNAR1 is highly expressed in leukocyte. So, it is likely that anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2020-04-30
Question
Would P00306 anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
It shows on the product datasheet, P00306 anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-22
Question
We are currently using anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody (P00306) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
Is this P00306 anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody reactive to the isotypes of IFNAR1?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The immunogen of P00306 anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of human interferon-alpha receptor, type I, subunit I (IFNAR1). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-05
Question
I was wanting to use your anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody for WB for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
As indicated on the product datasheet, P00306 anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-11
Question
I was wanting to use using your anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody for response to virus studies. Has this antibody been tested with western blotting on hek 293 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
I appreciate your inquiry. This P00306 anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody is tested on hek 293 cells. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-05
Question
I am looking for to test anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 on human leukocyte for research purposes, then I may be interested in using anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Walker
Verified customer
Asked: 2019-02-05
Answer
The products we sell, including anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-05
Question
We have seen staining in human myeloma. Are there any suggestions? Is anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody supposed to stain myeloma positively?
R. Lewis
Verified customer
Asked: 2018-03-13
Answer
According to literature myeloma does express IFNAR1. According to Uniprot.org, IFNAR1 is expressed in leukocyte, lung, liver, brain ovary, myeloma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have IFNAR1 expression, here are a few articles citing expression in various tissues:
Brain, and Ovary, Pubmed ID: 15489334
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 10830953
Lung, Pubmed ID: 14702039
Myeloma, Pubmed ID: 8307198
Boster Scientific Support
Answered: 2018-03-13
Question
Do you have a BSA free version of anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 available?
M. Banerjee
Verified customer
Asked: 2017-12-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-11
Question
Is a blocking peptide available for product anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody (P00306)?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
We do provide the blocking peptide for product anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody (P00306). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-25
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306. Let me know if you need anything else.
J. Parker
Verified customer
Asked: 2016-01-19
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-01-19
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306. Please let me know if you require anything else.
T. Walker
Verified customer
Asked: 2015-12-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-12-04
Question
My question regarding product P00306, anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Z. Zhao
Verified customer
Asked: 2015-06-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00306 anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-06-23
Question
I see that the anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 works with WB, what is the protocol used to produce the result images on the product page?
F. Wu
Verified customer
Asked: 2015-05-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-05-05
Question
Does anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 work on pig WB with brain ovary?
R. Krishna
Verified customer
Asked: 2015-02-13
Answer
Our lab technicians have not tested anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody P00306 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-13
Question
My team were happy with the WB result of your anti-Phospho-Interferon-alpha Receptor, Type I, Subunit 1 (Ser535,539) antibody. However we have been able to see positive staining in liver isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is IFNAR1 supposed to be expressed?
C. Zhang
Verified customer
Asked: 2014-04-29
Answer
From literature, liver does express IFNAR1. Generally IFNAR1 expresses in isoform 1: cell membrane. Regarding which tissues have IFNAR1 expression, here are a few articles citing expression in various tissues:
Brain, and Ovary, Pubmed ID: 15489334
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 10830953
Lung, Pubmed ID: 14702039
Myeloma, Pubmed ID: 8307198
Boster Scientific Support
Answered: 2014-04-29