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Product Info Summary
| SKU: | M00393 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Interferon gamma (IFNG) Monoclonal Antibody
SKU/Catalog Number
M00393
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Interferon gamma (IFNG) Monoclonal Antibody (Catalog # M00393). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Interferon gamma (IFNG) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00393)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: IFNG/466
Isotype
IgG2b, kappa
Immunogen
Purified recombinant human full-length IFNG protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00393 is reactive to IFNG in Human
Applications
M00393 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
19.348kDa
Background of IFN-gamma
Interferon (IFN)- translated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Reconstitute with 1ml water (15 minutes at room temperature).
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Anti-Interferon gamma Mouse Monoclonal Antibody (IFNG/466).
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Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Anti-Interferon gamma Mouse Monoclonal Antibody (IFNG/466).
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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Anti-Interferon gamma Mouse Monoclonal Antibody (IFNG/466).
Protein Target Info & Infographic
Gene/Protein Information For IFNG (Source: Uniprot.org, NCBI)
Gene Name
IFNG
Full Name
Interferon gamma
Weight
19.348kDa
Superfamily
type II (or gamma) interferon family
Alternative Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IFNG, check out the IFNG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interferon gamma (IFNG) Monoclonal Antibody (M00393)
Hello CJ!
M00393 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion
Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques
Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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4 Customer Q&As for Anti-Interferon gamma (IFNG) Monoclonal Antibody
Question
We have observed staining in human blood. Do you have any suggestions? Is anti-Interferon gamma (IFNG) Monoclonal antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
From what I have seen in literature blood does express IFNG. From what I have seen in Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-06
Question
We have tried in the past anti-Interferon gamma (IFNG) Monoclonal antibody for IF on blood a few months ago. I am using human, and We are going to use the antibody for IHC next. Our lab want to know about examining blood as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
I have checked the website and datasheets of our anti-Interferon gamma (IFNG) Monoclonal antibody and it appears that M00393 has been validated on human in both IF and IHC. Thus M00393 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-10-04
Question
We are currently using anti-Interferon gamma (IFNG) Monoclonal antibody M00393 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
L. Banerjee
Verified customer
Asked: 2015-01-15
Answer
The anti-Interferon gamma (IFNG) Monoclonal antibody (M00393) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-15
Question
My boss were well pleased with the WB result of your anti-Interferon gamma (IFNG) Monoclonal antibody. However we have observed positive staining in leukocyte secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?
B. Parker
Verified customer
Asked: 2014-01-24
Answer
From what I have seen in literature, leukocyte does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-01-24
