Anti-Iporin RUSC2 Antibody

Product Name

Anti-Iporin RUSC2 Antibody

View all RUSC2 Antibodies

SKU/Catalog Number

A12113-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Iporin RUSC2 Antibody (Catalog # A12113-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

RUSC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Iporin RUSC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12113-1)

Host

Rabbit

Contents

RUSC2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

RUSC2 antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human RUSC2. The immunogen is located within amino acids 70 - 120 of RUSC2.

Cross-reactivity

At least three isoforms are known to exist; this antibody will detect all three isoforms. This antibody is predicted to not cross-react with RUSC1.

Reactive Species

A12113-1 is reactive to RUSC2 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

161.2 kDa

Background of RUSC2

RUSC2, also known as Iporin, shares with the related protein RUSC1 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% amino acid identity. RUSC2 is a rab1-interacting protein that also interacts with GM130, another rab1-interacting protein. RUSC2 interacts with specific rab1 isoforms with different rab-binding specificity. It has been suggested that RUSC2 may function as a link between the targeting of ER derived vesicles triggered by the rab1 GTPase and a signaling pathway composed of proteins containing SH3 and/or poly-proline regions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A12113-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

RUSC2 antibody can be used for detection of RUSC2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information RUSC2

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis