Anti-IQSEC1 Antibody

Product Name

Anti-IQSEC1 Antibody

View all IQSEC1 Antibodies

SKU/Catalog Number

A06482

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-IQSEC1 Antibody (Catalog # A06482). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

IQSEC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-IQSEC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06482)

Host

Rabbit

Contents

IQSEC1 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1. The immunogen is located within amino acids 60 - 110 of IQSEC1.

Cross-reactivity

IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.

Reactive Species

A06482 is reactive to IQSEC1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

108.3 kDa

Background of IQSEC1

ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1). As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3). IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06482 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information IQSEC1

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