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Product Info Summary
| SKU: | PA2183 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IRAK/IRAK1 Antibody
SKU/Catalog Number
PA2183
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IRAK/IRAK1 Antibody catalog # PA2183. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRAK/IRAK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2183)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human IRAK, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2183 is reactive to IRAK1 in Human, Mouse, Rat
Applications
PA2183 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
274 kDa
Calculated molecular weight
76.537kDa
Background of IRAK1
Interleukin-1 receptor-associated kinase 1, also called IRAK1, is an enzyme that in humans is encoded by the IRAK1 gene. By radiation hybrid analysis, this gene is mapped to chromosome Xq28. Serine/threonine-protein kinase plays a critical role in initiating innate immune response against foreign pathogens. This gene involved in Toll-like receptor (TLR) and IL-1R signaling pathways. This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-IRAK antibody, PA2183, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: PANC Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For IRAK1 (Source: Uniprot.org, NCBI)
Gene Name
IRAK1
Full Name
Interleukin-1 receptor-associated kinase 1
Weight
76.537kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; Il1rak; interleukin-1 receptor-associated kinase 1; IRAK1; IRAK-1; IRAKPelle homolog; Pelle; Plpk IRAK1 IRAK, pelle interleukin 1 receptor associated kinase 1 interleukin-1 receptor-associated kinase 1|IRAK-1|Pelle homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRAK1, check out the IRAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRAK/IRAK1 Antibody (PA2183)
Hello CJ!
PA2183 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-IRAK/IRAK1 Antibody
Question
We are currently using anti-IRAK/IRAK1 antibody PA2183 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The anti-IRAK/IRAK1 antibody (PA2183) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-08
Question
Would anti-IRAK/IRAK1 antibody PA2183 work on dog WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not tested anti-IRAK/IRAK1 antibody PA2183 on dog. You can run a BLAST between dog and the immunogen sequence of anti-IRAK/IRAK1 antibody PA2183 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
My question regarding product PA2183, anti-IRAK/IRAK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2183 anti-IRAK/IRAK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-05
Question
Will PA2183 anti-IRAK/IRAK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Miller
Verified customer
Asked: 2014-04-11
Answer
You can see on the product datasheet, PA2183 anti-IRAK/IRAK1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-04-11
