Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3)

Boster Bio Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3) catalog # M00268-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00268-1
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3)

See all IRS1 products

SKU/Catalog Number

M00268-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3) catalog # M00268-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00268-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

10I3

Isotype

IgG2a

Immunogen

E.coli-derived human IRS1 recombinant protein (Position: S1041-Q1242). Human IRS1 shares 78% and 80% amino acid (aa) sequence identity with mouse and rat IRS1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00268-1 is reactive to IRS1 in Human

Applications

M00268-1 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For IRS1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P35568

Gene ID

3667

Gene Name

IRS1

Full Name

Insulin receptor substrate 1

Weight

131.591kDa

Alternative Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1 IRS1 HIRS-1 insulin receptor substrate 1 insulin receptor substrate 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IRS1, check out the IRS1 Infographic

IRS1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IRS1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M00268-1

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-IRS1 Antibody Picoband™ (monoclonal, 10I3)

Question

We have been able to see staining in human cervix carcinoma. What should we do? Is anti-IRS1 antibody (monoclonal, 10I3) supposed to stain cervix carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

From what I have seen in literature cervix carcinoma does express IRS1. From what I have seen in Uniprot.org, IRS1 is expressed in endometrium, skeletal muscle, eye, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have IRS1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 8513971

Boster Scientific Support

Answered: 2019-11-14

Question

Our lab used your anti-IRS1 antibody (monoclonal, 10I3) for IHC-P on endometrium in the past. I am using human, and We are going to use the antibody for WB next. We are interested in examining endometrium as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

I have checked the website and datasheets of our anti-IRS1 antibody (monoclonal, 10I3) and it appears that M00268-1 has been validated on human in both IHC-P and WB. Thus M00268-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-12

Question

We are currently using anti-IRS1 antibody (monoclonal, 10I3) M00268-1 for human tissue, and we are happy with the IHC-P results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-15

Answer

The anti-IRS1 antibody (monoclonal, 10I3) (M00268-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-15

Question

We are interested in using your anti-IRS1 antibody (monoclonal, 10I3) for positive regulation of fatty acid beta-oxidation studies. Has this antibody been tested with western blotting on sw620 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-06-15

Answer

We appreciate your inquiry. This M00268-1 anti-IRS1 antibody (monoclonal, 10I3) is tested on human hela, a549 whole cell lysates, sw620 whole cell lysates, hela whole cell lysates, u20s cells. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-06-15

$280

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
M00268-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.