Anti-IRS1 Rabbit Monoclonal Antibody

IRS1 antibody

Boster Bio Anti-IRS1 Rabbit Monoclonal Antibody catalog # M00268. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Product Info Summary

SKU: M00268
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-IRS1 Rabbit Monoclonal Antibody

View all IRS1 Antibodies

SKU/Catalog Number

M00268

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-IRS1 Rabbit Monoclonal Antibody catalog # M00268. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IRS1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00268)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

GIF-9

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human IRS1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00268 is reactive to IRS1 in Human

Applications

M00268 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

131.591kDa

Background of IRS1

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For IRS1 (Source: Uniprot.org, NCBI)

Gene Name

IRS1

Full Name

Insulin receptor substrate 1

Weight

131.591kDa

Alternative Names

HIRS-1; insulin receptor substrate 1; IRS1; IRS-1 IRS1 HIRS-1 insulin receptor substrate 1 insulin receptor substrate 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IRS1, check out the IRS1 Infographic

IRS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IRS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-IRS1 Rabbit Monoclonal Antibody

Question

We are currently using anti-IRS1 Rabbit Monoclonal antibody M00268 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

The anti-IRS1 Rabbit Monoclonal antibody (M00268) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-04

Question

We bought anti-IRS1 Rabbit Monoclonal antibody for WB on eye a few years ago. I am using human, and We intend to use the antibody for IF next. We are interested in examining eye as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

D. Williams

Verified customer

Asked: 2019-11-22

Answer

I took a look at the website and datasheets of our anti-IRS1 Rabbit Monoclonal antibody and it appears that M00268 has been validated on human in both WB and IF. Thus M00268 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-22

Question

We have observed staining in human skeletal muscle. Any tips? Is anti-IRS1 Rabbit Monoclonal antibody supposed to stain skeletal muscle positively?

Verified Customer

Verified customer

Asked: 2018-02-01

Answer

According to literature skeletal muscle does express IRS1. According to Uniprot.org, IRS1 is expressed in endometrium, skeletal muscle, eye, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have IRS1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 8513971

Boster Scientific Support

Answered: 2018-02-01

Order DetailsPrice
M00268

100μL

$370
M00268-30ul

30μL sample

$99
M00268-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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