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Product Info Summary
| SKU: | M00268 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-IRS1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00268
BM4666 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IRS1 Rabbit Monoclonal Antibody catalog # M00268. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRS1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00268)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GIF-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IRS1
Reactive Species
M00268 is reactive to IRS1 in Human
Observed Molecular Weight
170 kDa
Calculated molecular weight
131.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00268 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: HEK293 cell
Validation Images & Assay Conditions
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Western blot analysis of IRS1 expression in HEK293 cell lysate.
Specific Publications For Anti-IRS1 Rabbit Monoclonal Antibody (M00268)
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3 Customer Q&As for Anti-IRS1 Rabbit Monoclonal Antibody
Question
We are currently using anti-IRS1 Rabbit Monoclonal antibody M00268 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
The anti-IRS1 Rabbit Monoclonal antibody (M00268) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-04
Question
We bought anti-IRS1 Rabbit Monoclonal antibody for WB on eye a few years ago. I am using human, and We intend to use the antibody for IF next. We are interested in examining eye as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
D. Williams
Verified customer
Asked: 2019-11-22
Answer
I took a look at the website and datasheets of our anti-IRS1 Rabbit Monoclonal antibody and it appears that M00268 has been validated on human in both WB and IF. Thus M00268 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-22
Question
We have observed staining in human skeletal muscle. Any tips? Is anti-IRS1 Rabbit Monoclonal antibody supposed to stain skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
According to literature skeletal muscle does express IRS1. According to Uniprot.org, IRS1 is expressed in endometrium, skeletal muscle, eye, cervix carcinoma, leukemic t-cell, liver, among other tissues. Regarding which tissues have IRS1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Skeletal muscle, Pubmed ID: 8513971
Boster Scientific Support
Answered: 2018-02-01
