Anti-ISL2/Islet 2 Antibody

Islet-2 antibody

Boster Bio Anti-ISL2/Islet 2 Antibody (Catalog # A10787). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A10787
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-ISL2/Islet 2 Antibody

View all Islet-2 Antibodies

SKU/Catalog Number

A10787

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-ISL2/Islet 2 Antibody (Catalog # A10787). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ISL2/Islet 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10787)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human ISL2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10787 is reactive to Isl2 in Human, Mouse, Rat

Applications

A10787 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

39.675kDa

Background of Islet-2

Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways By similarity.

The MGC Project Team, Genome Res. 14:2121-2127(2004).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Isl2 (Source: Uniprot.org, NCBI)

Gene Name

Isl2

Full Name

Insulin gene enhancer protein ISL-2

Weight

39.675kDa

Alternative Names

FLJ10160; insulin gene enhancer protein ISL-2; ISL LIM homeobox 2; ISL2 transcription factor, LIM/homeodomain, (islet-2); ISL2; Islet2; Islet-2 Isl2|ISL LIM homeobox 2|insulin gene enhancer protein ISL-2|insulin related protein 2 (islet 2)|islet-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Isl2, check out the Isl2 Infographic

Isl2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Isl2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ISL2/Islet 2 Antibody

Question

Would anti-ISL2/Islet 2 antibody A10787 work on horse WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

Our lab technicians have not validated anti-ISL2/Islet 2 antibody A10787 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ISL2/Islet 2 antibody A10787 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-29

Question

Is there a BSA free version of anti-ISL2/Islet 2 antibody A10787 available?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ISL2/Islet 2 antibody A10787 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-12-05

Question

See attached the WB image, lot number and protocol we used for ectocervix using anti-ISL2/Islet 2 antibody A10787. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-29

Question

Is this A10787 anti-ISL2/Islet 2 antibody reactive to the isotypes of ISL2?

Verified Customer

Verified customer

Asked: 2019-04-24

Answer

The immunogen of A10787 anti-ISL2/Islet 2 antibody is Synthesized peptide derived from internal of human ISL2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-04-24

Question

We are currently using anti-ISL2/Islet 2 antibody A10787 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?

C. Thomas

Verified customer

Asked: 2018-03-27

Answer

The anti-ISL2/Islet 2 antibody (A10787) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-27

Question

I see that the anti-ISL2/Islet 2 antibody A10787 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-09-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-09-05

Question

Does anti-ISL2/Islet 2 antibody A10787 work for WB with ectocervix?

D. Roberts

Verified customer

Asked: 2013-02-19

Answer

According to the expression profile of ectocervix, ISL2 is highly expressed in ectocervix. So, it is likely that anti-ISL2/Islet 2 antibody A10787 will work for WB with ectocervix.

Boster Scientific Support

Answered: 2013-02-19

Order DetailsPrice
A10787-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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