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Product Info Summary
| SKU: | A06444-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Semaphorin 3c/SEMA3C Antibody Picoband®
SKU/Catalog Number
A06444-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Semaphorin 3c/SEMA3C Antibody Picoband® catalog # A06444-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Semaphorin 3c/SEMA3C Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06444-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Semaphorin 3c/SEMA3C recombinant protein (Position: Q24-W674).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06444-1 is reactive to SEMA3C in Human, Mouse, Rat
Observed Molecular Weight
85 kDa
Calculated molecular weight
85.2 kDa
Background of SEMA3C
Semaphorin-3C is a protein that in humans is encoded by the SEMA3C gene. This gene encodes a secreted glycoprotein that belongs to the semaphorin class 3 family of neuronal guidance cues. The encoded protein contains an N-terminal sema domain, integrin and immunoglobulin-like domains, and a C-terminal basic domain. Homodimerization and proteolytic cleavage of the C-terminal propeptide are necessary for the function of the encoded protein. It binds a neuropilin co-receptor before forming a heterotrimeric complex with an associated plexin. An increase in the expression of this gene correlates with an increase in cancer cell invasion and adhesion. Naturally occurring mutations in this gene are associated with Hirschsprung disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06444-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissues, human A549 whole cell, human U2OS whole cell, rat liver tissue, mouse lung tissue, mouse kidney tissue, mouse liver tissue,
Validation Images & Assay Conditions
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Western blot analysis of SEMA3C using anti-SEMA3C antibody (A06444-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissues lysates
Lane 2: human A549 whole cell lysates
Lane 3: human U2OS whole cell lysates
Lane 4: rat liver tissue lysates
Lane 5: mouse lung tissue lysates
Lane 6: mouse kidney tissue lysates
Lane 7: mouse liver tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3C antigen affinity purified polyclonal antibody (Catalog # A06444-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3C at approximately 85KD. The expected band size for SEMA3C is at 85KD.
Specific Publications For Anti-Semaphorin 3c/SEMA3C Antibody Picoband® (A06444-1)
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4 Customer Q&As for Anti-Semaphorin 3c/SEMA3C Antibody Picoband®
Question
Will anti-Semaphorin 3c/SEMA3C antibody A06444-1 work for WB with testis?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
According to the expression profile of testis, SEMA3C is highly expressed in testis. So, it is likely that anti-Semaphorin 3c/SEMA3C antibody A06444-1 will work for WB with testis.
Boster Scientific Support
Answered: 2020-02-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Semaphorin 3c/SEMA3C antibody A06444-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-14
Question
We are currently using anti-Semaphorin 3c/SEMA3C antibody A06444-1 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The anti-Semaphorin 3c/SEMA3C antibody (A06444-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-05
Question
My question regarding product A06444-1, anti-Semaphorin 3c/SEMA3C antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06444-1 anti-Semaphorin 3c/SEMA3C antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-05
