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Product Info Summary
| SKU: | A04689 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-JMJD2C Antibody (C-term)
View all Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C Antibodies
SKU/Catalog Number
A04689
Size
80 µl
Description
Boster Bio Anti-JMJD2C Antibody (C-term) (Catalog # A04689). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-JMJD2C Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04689)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This JMJD2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1023-1056 amino acids from the C-terminal region of human JMJD2C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04689 is reactive to KDM4C in Human, Mouse
Reconstitution
Calculated molecular weight
119982 Da
Background of Lysine (K)-specific Demethylase 4C/KDM4C/JMJD2C
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein with one JmjC domain, one JmjN domain, two PHD-type zinc fingers, and two Tudor domains. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form. Chromosomal aberrations and increased transcriptional expression of this gene are associated with esophageal squamous cell carcinoma. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04689 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-JMJD2C Antibody (C-term) at 1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: K562 whole cell lysates
Lane 3: LNCaP whole cell lysates
Lane 4: mouse brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 120 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with A04689 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A04689, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Click image to see more details
Overlay histogram showing Hela cells stained with A04689 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A04689, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For KDM4C (Source: Uniprot.org, NCBI)
Gene Name
KDM4C
Full Name
Lysine-specific demethylase 4C
Weight
119982 Da
Superfamily
JHDM3 histone demethylase family
Alternative Names
Lysine-specific demethylase 4C, 11411-, Gene amplified in squamous cell carcinoma 1 protein, GASC-1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, KDM4C, GASC1, JHDM3C, JMJD2C, KIAA0780 KDM4C GASC1, JHDM3C, JMJD2C, TDRD14C lysine demethylase 4C lysine-specific demethylase 4C|JmjC domain-containing histone demethylation protein 3C|[histone H3]-trimethyl-L-lysine(9) demethylase 4C|gene amplified in squamous cell carcinoma 1 protein|jumonji domain-containing protein 2C|lysine (K)-specific demethylase 4C|tudor domain containing 14C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KDM4C, check out the KDM4C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KDM4C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JMJD2C Antibody (C-term) (A04689)
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