Click image to see more details
Product Info Summary
| SKU: | M02608-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02608-1
BM4329 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody catalog # M02608-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02608-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DFI-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human JNK1+JNK2+JNK3
Reactive Species
M02608-1 is reactive to MAPK8 in Human, Mouse, Rat
Observed Molecular Weight
40, 48 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02608-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human U87 whole cell, human A549 whole cell, rat PC-12 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of JNK1/2/3 using anti-JNK1/2/3 antibody (M02608-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK1/2/3 antigen affinity purified monoclonal antibody (Catalog # M02608-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK1/2/3 at approximately 40, 48 kDa. The expected band size for JNK1/2/3 is at 48 kDa.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Click image to see more details
Immunofluorescent analysis of Hela cells, using JNK1/2/3 Antibody.
Click image to see more details
Effects of OPN and OVE on activation of MAPK signaling pathways in LPS-stimulated RAW264.7 cells. Expression levels of p-ERK, ERK, p-p38, p-38, p-JNK, and JNK were detected in the same samples for COX-2 detection after 24 h of LPS stimulation. (A) OVE treatment. (B) OPN treatment. All experiments were carried out in triplicates and data are presented as means ± SDs; one-way ANOVA analysis was adopted for multiple comparisons; ###P < 0.001, ####P < 0.0001, compared to the untreated control group; ***P < 0.001 and ****P < 0.0001, compared to the LPS control group.
Index in PubMed under a CC BY license. PMID: 39455284
Specific Publications For Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody (M02608-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-JNK1/2/3 MAPK8 Rabbit Monoclonal Antibody
Question
Is this M02608-1 anti-JNK1/2/3 Rabbit Monoclonal antibody reactive to the isotypes of MAPK8?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The immunogen of M02608-1 anti-JNK1/2/3 Rabbit Monoclonal antibody is A synthesized peptide derived from human JNK1+JNK2+JNK3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-25
Question
I was wanting to use using your anti-JNK1/2/3 Rabbit Monoclonal antibody for activation of bmf and translocation to mitochondria studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
We appreciate your inquiry. This M02608-1 anti-JNK1/2/3 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IP, IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-03-24
Question
I see that the anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-02
Question
Will M02608-1 anti-JNK1/2/3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can see on the product datasheet, M02608-1 anti-JNK1/2/3 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-03
Question
Do you have a BSA free version of anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1 available?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-01
Question
Would anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1 work for IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-04-19
Answer
According to the expression profile of cervix carcinoma, MAPK8 is highly expressed in cervix carcinoma. So, it is likely that anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2019-04-19
Question
We are currently using anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
The anti-JNK1/2/3 Rabbit Monoclonal antibody (M02608-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1. Let me know if you need anything else.
T. Carter
Verified customer
Asked: 2019-01-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-14
Question
I was wanting to use your anti-JNK1/2/3 Rabbit Monoclonal antibody for IF for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-04-26
Answer
You can see on the product datasheet, M02608-1 anti-JNK1/2/3 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-26
Question
Is a blocking peptide available for product anti-JNK1/2/3 Rabbit Monoclonal antibody (M02608-1)?
P. Wu
Verified customer
Asked: 2018-03-23
Answer
We do provide the blocking peptide for product anti-JNK1/2/3 Rabbit Monoclonal antibody (M02608-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-03-23
Question
My team were well pleased with the WB result of your anti-JNK1/2/3 Rabbit Monoclonal antibody. However we have observed positive staining in forebrain cytoplasm. using this antibody. Is that expected? Could you tell me where is MAPK8 supposed to be expressed?
O. Jha
Verified customer
Asked: 2018-03-01
Answer
From what I have seen in literature, forebrain does express MAPK8. Generally MAPK8 expresses in cytoplasm. Regarding which tissues have MAPK8 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8654373
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Fetal brain, Pubmed ID: 8137421
Boster Scientific Support
Answered: 2018-03-01
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-29
Question
We have been able to see staining in rat fetal brain. Any tips? Is anti-JNK1/2/3 Rabbit Monoclonal antibody supposed to stain fetal brain positively?
B. Rodriguez
Verified customer
Asked: 2018-01-08
Answer
According to literature fetal brain does express MAPK8. According to Uniprot.org, MAPK8 is expressed in forebrain, fetal brain, brain, cervix carcinoma, among other tissues. Regarding which tissues have MAPK8 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8654373
Cervix carcinoma, Pubmed ID: 18669648, 18691976
Fetal brain, Pubmed ID: 8137421
Boster Scientific Support
Answered: 2018-01-08
Question
Our lab want to know about to test anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Parker
Verified customer
Asked: 2017-07-27
Answer
The products we sell, including anti-JNK1/2/3 Rabbit Monoclonal antibody M02608-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-27
Question
Can you help my question with product M02608-1, anti-JNK1/2/3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Li
Verified customer
Asked: 2015-08-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02608-1 anti-JNK1/2/3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-08-03
Question
We bought anti-JNK1/2/3 Rabbit Monoclonal antibody for IP on fetal brain a few months ago. I am using rat, and We intend to use the antibody for WB next. I am looking for examining fetal brain as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
W. Miller
Verified customer
Asked: 2013-05-17
Answer
I have checked the website and datasheets of our anti-JNK1/2/3 Rabbit Monoclonal antibody and it appears that M02608-1 has been tested on rat in both IP and WB. Thus M02608-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-05-17
