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- Table of Contents
MAPK8 antibody (M02608-3) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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IHC analysis of JNK1/MAPK8 using anti-JNK1/MAPK8 antibody (M02608-3).
JNK1/MAPK8 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JNK1/MAPK8 Antibody (M02608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
JNK1/MAPK8 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-JNK1/MAPK8 Antibody (M02608-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-JNK1 Rabbit Monoclonal Antibody
MAPK8 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
MAPK8 antibody was positively detected by WB in:
human 293T whole cell, human Hela whole cell, human Jurkat whole cell, human A431 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
MAPK8 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue, human prostate cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-JNK1 Rabbit Monoclonal Antibody
View all MAPK8 Antibodies
View all MAPK8 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
29M34
Isotype
IgG
Immunogen
A synthesized peptide derived from human JNK1
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated Molecular Weight
48.3 kDa
Cite This Product
Anti-JNK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02608-3)
Protocols
Western Blot protocol for MAPK8 antibody
Immunohistochemistry (IHC) protocol for MAPK8 antibody
Publications
Application:
Species:
Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice
Published: 2022-12-02
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 6.21
Species: Mouse
PMID: 36499473
Gut microbiota modulates osteoclast glutathione synthesis and mitochondrial biogenesis in mice subjected to ovariectomy
Published: 2022-01-26
Journal: CELL PROLIFERATION
Application: WB
Impact Factor: 8.76
Species: Mouse
PMID: 35080066
Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential
Published: 2020-08-14
Journal: JOURNAL OF FOOD SCIENCE
Application: WB
Impact Factor: 2.48
Species: Mouse
PMID: 32794226
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Product Promise
We promise Anti-JNK1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M02608-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026