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Product Info Summary
| SKU: | M04297 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-JNK3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04297
BM4743 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-JNK3 Rabbit Monoclonal Antibody catalog # M04297. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JNK3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04297)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HGC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human JNK3
Reactive Species
M04297 is reactive to MAPK10 in Human, Mouse, Rat
Observed Molecular Weight
50,53 kDa
Calculated molecular weight
52.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04297 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
Positive Control
WB: human Hela whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of JNK3 using anti-JNK3 antibody (M04297).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK3 antigen affinity purified monoclonal antibody (Catalog # M04297) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JNK3 at approximately 50,53 kDa. The expected band size for JNK3 is at 53 kDa.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Specific Publications For Anti-JNK3 Rabbit Monoclonal Antibody (M04297)
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6 Customer Q&As for Anti-JNK3 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-JNK3 Rabbit Monoclonal antibody for WB for rat frontal cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat frontal cortex identification?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
It shows on the product datasheet, M04297 anti-JNK3 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-29
Question
Will anti-JNK3 Rabbit Monoclonal antibody M04297 work on canine IF with hippocampus?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
Our lab technicians have not tested anti-JNK3 Rabbit Monoclonal antibody M04297 on canine. You can run a BLAST between canine and the immunogen sequence of anti-JNK3 Rabbit Monoclonal antibody M04297 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine hippocampus in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-08
Question
Would M04297 anti-JNK3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
It shows on the product datasheet, M04297 anti-JNK3 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-01
Question
Would anti-JNK3 Rabbit Monoclonal antibody M04297 work for WB with frontal cortex?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
According to the expression profile of frontal cortex, MAPK10 is highly expressed in frontal cortex. So, it is likely that anti-JNK3 Rabbit Monoclonal antibody M04297 will work for WB with frontal cortex.
Boster Scientific Support
Answered: 2017-08-30
Question
Please see the WB image, lot number and protocol we used for frontal cortex using anti-JNK3 Rabbit Monoclonal antibody M04297. Please let me know if you require anything else.
E. Johnson
Verified customer
Asked: 2015-04-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for frontal cortex using anti-JNK3 Rabbit Monoclonal antibody M04297. Let me know if you need anything else.
R. Jackson
Verified customer
Asked: 2013-12-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-02
