Anti-JunB (Ab-79) Antibody

Product Name

Anti-JunB (Ab-79) Antibody

View all JunB/AP-1 Antibodies

SKU/Catalog Number

A01825

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-JunB (Ab-79) Antibody (Catalog # A01825). Tested in IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-JunB (Ab-79) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01825)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa.77~81 (G-A-S-L-K) derived from Human JunB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01825 is reactive to JUNB in Human

Applications

A01825 is guaranteed for IF, IHC Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

17935 MW

Background of JunB/AP-1

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Narayanan K, et al. (2004) J Biol Chem. 279(43): 44294-442302.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 43kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

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Figure 1. IHC analysis of JUNB using anti-JUNB antibody (A01825).
JUNB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-JUNB Antibody (A01825) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Immunofluorescence staining of methanol-fixed Hela cells using JunB(Ab-79) Antibody #A01825.

Gene/Protein Information For JUNB (Source: Uniprot.org, NCBI)

Alternative Names

activator protein 1; AP-1; jun B proto-oncogene; JunB; transcription factor jun-B JUNB AP-1 JunB proto-oncogene, AP-1 transcription factor subunit transcription factor jun-B|activator protein 1|jun B proto-oncogene

For more info on JUNB, check out the JUNB Infographic

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In this infographic, you will see the following information for JUNB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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