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Product Info Summary
| SKU: | M01825 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-JunB Rabbit Monoclonal Antibody
SKU/Catalog Number
M01825
BM4730 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-JunB Rabbit Monoclonal Antibody catalog # M01825. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JunB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01825)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HFO-10
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human JunB
Reactive Species
M01825 is reactive to JUNB in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
35.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01825 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
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Western blot analysis of JunB expression in HeLa cell lysate.
Specific Publications For Anti-JunB Rabbit Monoclonal Antibody (M01825)
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Customer Q&As
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7 Customer Q&As for Anti-JunB Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-JunB Rabbit Monoclonal antibody for IP for rat cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
You can see on the product datasheet, M01825 anti-JunB Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-10
Question
Is this M01825 anti-JunB Rabbit Monoclonal antibody reactive to the isotypes of JUNB?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The immunogen of M01825 anti-JunB Rabbit Monoclonal antibody is A synthesized peptide derived from human JunB . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-30
Question
Is a blocking peptide available for product anti-JunB Rabbit Monoclonal antibody (M01825)?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
We do provide the blocking peptide for product anti-JunB Rabbit Monoclonal antibody (M01825). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-25
Question
I see that the anti-JunB Rabbit Monoclonal antibody M01825 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-12-17
Question
you antibody to test anti-JunB Rabbit Monoclonal antibody M01825 on rat cervix carcinoma for research purposes, then I may be interested in using anti-JunB Rabbit Monoclonal antibody M01825 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
The products we sell, including anti-JunB Rabbit Monoclonal antibody M01825, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-16
Question
My question regarding product M01825, anti-JunB Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Taylor
Verified customer
Asked: 2017-10-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01825 anti-JunB Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-06
Question
Does anti-JunB Rabbit Monoclonal antibody M01825 work on primate IP with tibial artery?
A. Huang
Verified customer
Asked: 2014-03-19
Answer
Our lab technicians have not tested anti-JunB Rabbit Monoclonal antibody M01825 on primate. You can run a BLAST between primate and the immunogen sequence of anti-JunB Rabbit Monoclonal antibody M01825 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate tibial artery in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-03-19
