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Product Info Summary
| SKU: | A08160 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Junctophilin-3 JPH3 Antibody
SKU/Catalog Number
A08160
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Junctophilin-3 JPH3 Antibody (Catalog # A08160). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
JPH3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Junctophilin-3 JPH3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08160)
Host
Rabbit
Contents
JPH3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
JPH3 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human JPH3. The immunogen is located within amino acids 540 - 590 of JPH3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of ZBTB4 are known to exist; this antibody will only recognize the longest isoform. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A08160 is reactive to JPH3 in Human, Mouse, Rat
Applications
A08160 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
81.469kDa
Background of JPH3
Junctional complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR) are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. Junctophilins (JPs) are important components of the junctional complexes. JPs are composed of a carboxy-terminal hydrophobic segment spanning the ER/SR membrane and a remaining cytoplasmic domain that shows specific affinity for the PM. Four JPs have been identified as tissue-specific subtypes derived from different genes: JPH1 is expressed in skeletal muscle, JPH2 is detected throughout all muscle cell types, and JPH3 and JPH4 are predominantly expressed in the brain. In the CNS, both JPH3 and JPH4 are expressed throughout neural sites and contribute to the subsurface cistern formation in neurons. Mice lacking both JPH3 and JPH4 subtypes exhibit serious symptoms such as impaired learning and memory and are accompanied by abnormal nervous functions. A repeat expansion in JPH3 is associated with Huntington disease-like 2. At least two isoforms of JPH3 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
JPH3 antibody can be used for detection of JPH3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of JPH3 in Daudi cell lysate with JPH3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of JPH3 in human brain tissue with JPH3 antibody at 2.5 μg/mL.
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Immunofluorescence of JPH3 in Human Brain cells with JPH3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For JPH3 (Source: Uniprot.org, NCBI)
Gene Name
JPH3
Full Name
Junctophilin-3
Weight
81.469kDa
Superfamily
junctophilin family
Alternative Names
CAGL237; junctophilin 3; junctophilin type 3 JPH3 CAGL237, HDL2, JP-3, JP3, TNRC22 junctophilin 3 junctophilin-3|junctophilin type 3|trinucleotide repeat containing 22
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JPH3, check out the JPH3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JPH3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Junctophilin-3 JPH3 Antibody (A08160)
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No publications found for A08160
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Customer Reviews
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