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Product Info Summary
| SKU: | M03058 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-PPP1R13L Antibody (N-Term)
SKU/Catalog Number
M03058
Size
80 µl
Description
Boster Bio Anti-PPP1R13L Antibody (N-Term) (Catalog # M03058). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PPP1R13L Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03058)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PPP1R13L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-166 amino acids from human PPP1R13L.
Reactive Species
M03058 is reactive to PPP1R13L in Human
Calculated molecular weight
89.1 kDa
Background of PPP1R13L
Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03058 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling PPP1R13L with M03058 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-PPP1R13L Antibody (N-Term) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysates
Lane 2: Hela whole cell lysates
Lane 3: NCI-H1299 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 89 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-PPP1R13L Antibody (N-Term) (M03058)
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