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Product Info Summary
| SKU: | M06353 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-K-Cadherin-6 CDH6-Monoclonal Antibody
SKU/Catalog Number
M06353
BM5697 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-K-Cadherin-6 CDH6-Monoclonal Antibody catalog # M06353. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-K-Cadherin-6 CDH6-Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06353)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFCE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human K Cadherin
Reactive Species
M06353 is reactive to CDH6 in Human, Mouse, Rat
Observed Molecular Weight
130 kDa
Calculated molecular weight
88.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06353 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Positive Control
WB: Jurkat cell, Mouse heart
Validation Images & Assay Conditions
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Western blot analysis of K Cadherin expression in (1) Jurkat cell lysate; (2) Mouse heart lysate.
Specific Publications For Anti-K-Cadherin-6 CDH6-Monoclonal Antibody (M06353)
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Customer Q&As
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6 Customer Q&As for Anti-K-Cadherin-6 CDH6-Monoclonal Antibody
Question
Hello, I would like to know which part of CDH6 is recognized by your antibody ? Is it the ectodomain or the intracellular domain ? Thanks Tom
Verified customer
Asked: 2022-07-07
Answer
Hi Tom, Thank you for your question. The immunogen sequence for M06353 is YLSDWGPRFKKLAD.
Boster Scientific Support
Answered: 2022-07-08
Question
We are currently using anti-K Cadherin Monoclonal antibody M06353 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The anti-K Cadherin Monoclonal antibody (M06353) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-30
Question
Does M06353 anti-K Cadherin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
It shows on the product datasheet, M06353 anti-K Cadherin Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-20
Question
I was wanting to use your anti-K Cadherin Monoclonal antibody for IP for mouse spinal cord on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse spinal cord identification?
A. Walker
Verified customer
Asked: 2020-01-20
Answer
You can see on the product datasheet, M06353 anti-K Cadherin Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
Will anti-K Cadherin Monoclonal antibody M06353 work for IP with spinal cord?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
According to the expression profile of spinal cord, CDH6 is highly expressed in spinal cord. So, it is likely that anti-K Cadherin Monoclonal antibody M06353 will work for IP with spinal cord.
Boster Scientific Support
Answered: 2019-08-23
Question
Will anti-K Cadherin Monoclonal antibody M06353 work on dog IP with plasma?
R. Taylor
Verified customer
Asked: 2016-08-24
Answer
Our lab technicians have not tested anti-K Cadherin Monoclonal antibody M06353 on dog. You can run a BLAST between dog and the immunogen sequence of anti-K Cadherin Monoclonal antibody M06353 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog plasma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-08-24
