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Product Info Summary
| SKU: | M06353 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-K-Cadherin-6 CDH6-Monoclonal Antibody
View all Cadherin-6/KCAD Antibodies
SKU/Catalog Number
M06353
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-K-Cadherin-6 CDH6-Monoclonal Antibody catalog # M06353. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-K-Cadherin-6 CDH6-Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06353)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFCE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human K Cadherin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06353 is reactive to CDH6 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
51 kDa
Calculated molecular weight
104738 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06353 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Positive Control
WB: Jurkat cell, Mouse heart
Validation Images & Assay Conditions
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Western blot analysis of K Cadherin expression in (1) Jurkat cell lysate; (2) Mouse heart lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDH6 (Source: Uniprot.org, NCBI)
Gene Name
CDH6
Full Name
Cadherin-6
Weight
104738 MW
Alternative Names
Type II inositol 3,4-bisphosphate 4-phosphatase;3.1.3.66;Inositol polyphosphate 4-phosphatase type II;INPP4B; CDH6 CAD6, KCAD cadherin 6 cadherin-6|cadherin 6, type 2, K-cadherin (fetal kidney)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH6, check out the CDH6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-K-Cadherin-6 CDH6-Monoclonal Antibody (M06353)
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No publications found for M06353
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-K-Cadherin-6 CDH6-Monoclonal Antibody
Question
Hello, I would like to know which part of CDH6 is recognized by your antibody ? Is it the ectodomain or the intracellular domain ? Thanks Tom
Verified customer
Asked: 2022-07-07
Answer
Hi Tom, Thank you for your question. The immunogen sequence for M06353 is YLSDWGPRFKKLAD.
Boster Scientific Support
Answered: 2022-07-08
Question
We are currently using anti-K Cadherin Monoclonal antibody M06353 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
The anti-K Cadherin Monoclonal antibody (M06353) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-30
Question
Does M06353 anti-K Cadherin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
It shows on the product datasheet, M06353 anti-K Cadherin Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-20
Question
I was wanting to use your anti-K Cadherin Monoclonal antibody for IP for mouse spinal cord on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse spinal cord identification?
A. Walker
Verified customer
Asked: 2020-01-20
Answer
You can see on the product datasheet, M06353 anti-K Cadherin Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
Will anti-K Cadherin Monoclonal antibody M06353 work for IP with spinal cord?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
According to the expression profile of spinal cord, CDH6 is highly expressed in spinal cord. So, it is likely that anti-K Cadherin Monoclonal antibody M06353 will work for IP with spinal cord.
Boster Scientific Support
Answered: 2019-08-23
Question
Will anti-K Cadherin Monoclonal antibody M06353 work on dog IP with plasma?
R. Taylor
Verified customer
Asked: 2016-08-24
Answer
Our lab technicians have not tested anti-K Cadherin Monoclonal antibody M06353 on dog. You can run a BLAST between dog and the immunogen sequence of anti-K Cadherin Monoclonal antibody M06353 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog plasma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-08-24
