Anti-K-Cadherin-6 CDH6-Monoclonal Antibody

Cadherin-6/KCAD antibody

Boster Bio Anti-K-Cadherin-6 CDH6-Monoclonal Antibody catalog # M06353. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M06353
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, WB

Product Name

Anti-K-Cadherin-6 CDH6-Monoclonal Antibody

View all Cadherin-6/KCAD Antibodies

SKU/Catalog Number

M06353

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-K-Cadherin-6 CDH6-Monoclonal Antibody catalog # M06353. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-K-Cadherin-6 CDH6-Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06353)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFCE-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human K Cadherin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M06353 is reactive to CDH6 in Human, Mouse, Rat

Applications

M06353 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

51 kDa

Calculated molecular weight

88.309kDa

Background of Cadherin-6/KCAD

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CDH6 (Source: Uniprot.org, NCBI)

Gene Name

CDH6

Full Name

Cadherin-6

Weight

88.309kDa

Alternative Names

cadherin 6, type 2, K-cadherin (fetal kidney); cadherin, fetal kidney; Cadherin6; Cadherin-6; CDH6; KCAD; K-cadherin (fetal kidney); K-Cadherin CDH6 CAD6, KCAD cadherin 6 cadherin-6|cadherin 6, type 2, K-cadherin (fetal kidney)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDH6, check out the CDH6 Infographic

CDH6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDH6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-K-Cadherin-6 CDH6-Monoclonal Antibody

Question

Hello, I would like to know which part of CDH6 is recognized by your antibody ? Is it the ectodomain or the intracellular domain ? Thanks Tom

Verified customer

Asked: 2022-07-07

Answer

Hi Tom, Thank you for your question. The immunogen sequence for M06353 is YLSDWGPRFKKLAD.

Boster Scientific Support

Answered: 2022-07-08

Question

We are currently using anti-K Cadherin Monoclonal antibody M06353 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

The anti-K Cadherin Monoclonal antibody (M06353) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-30

Question

Does M06353 anti-K Cadherin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

It shows on the product datasheet, M06353 anti-K Cadherin Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-20

Question

I was wanting to use your anti-K Cadherin Monoclonal antibody for IP for mouse spinal cord on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse spinal cord identification?

A. Walker

Verified customer

Asked: 2020-01-20

Answer

You can see on the product datasheet, M06353 anti-K Cadherin Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse spinal cord in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-20

Question

Will anti-K Cadherin Monoclonal antibody M06353 work for IP with spinal cord?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

According to the expression profile of spinal cord, CDH6 is highly expressed in spinal cord. So, it is likely that anti-K Cadherin Monoclonal antibody M06353 will work for IP with spinal cord.

Boster Scientific Support

Answered: 2019-08-23

Question

Will anti-K Cadherin Monoclonal antibody M06353 work on dog IP with plasma?

R. Taylor

Verified customer

Asked: 2016-08-24

Answer

Our lab technicians have not tested anti-K Cadherin Monoclonal antibody M06353 on dog. You can run a BLAST between dog and the immunogen sequence of anti-K Cadherin Monoclonal antibody M06353 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog plasma in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-08-24

Order DetailsPrice
M06353

100μL

$370
M06353-30ul

30μL sample

$99
M06353-carrier-free

Carrier Free

$370

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