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Product Info Summary
| SKU: | A08426 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Kallistatin/PI-4/SERPINA4 Antibody Picoband®
SKU/Catalog Number
A08426
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallistatin/PI-4/SERPINA4 Antibody Picoband® catalog # A08426. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallistatin/PI-4/SERPINA4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08426)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Kallistatin/PI-4/SERPINA4 recombinant protein (Position: H147-A373).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08426 is reactive to SERPINA4 in Human, Monkey, Rat
Observed Molecular Weight
49 kDa
Calculated molecular weight
48.5 kDa
Background of SERPINA4
Kallistatin is a protein that in humans is encoded by the SERPINA4 gene. It is mapped to 14q32.13. Serpin A4 is a member of the Serpin superfamily of the serine protease inhibitors. Known also as Kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08426 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Western blot analysis of SERPINA4 using anti-SERPINA4 antibody (A08426).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: monkey liver tissue lysates,
Lane 4: monkey liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA4 antigen affinity purified polyclonal antibody (Catalog # A08426) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA4 at approximately 49KD. The expected band size for SERPINA4 is at 49KD.
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IHC analysis of SERPINA4 using anti-SERPINA4 antibody (A08426).
SERPINA4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SERPINA4 Antibody (A08426) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Kallistatin/PI-4/SERPINA4 Antibody Picoband® (A08426)
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