Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody

Product Name

Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody

View all Kappa Light Chain Antibodies

SKU/Catalog Number

M05470

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody (Catalog # M05470). Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Kappa Light Chain (B-Cell Marker) IGKV1D-16 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05470)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM508

Isotype

IgG1, kappa

Immunogen

Human B-Lymphoma Cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M05470 is reactive to IGKC in Human

Applications

M05470 is guaranteed for Flow Cytometry, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

11.765kDa

Background of Kappa Light Chain

This monoclonal antibody is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (0.5-2.0ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-Kappa Light Chain Monoclonal Antibody (SPM508).

Gene/Protein Information For IGKC (Source: Uniprot.org, NCBI)

Alternative Names

HCAK1; IGKCD; immunoglobulin kappa constant; Km; MGC111575; MGC62011; MGC72072; MGC88770; MGC88771; MGC88809 IGKC HCAK1D, Km, IGKC immunoglobulin kappa constant immunoglobulin kappa (invariant region)|immunoglobulin kappa constant region|immunoglobulin kappa light chain (VJ)

For more info on IGKC, check out the IGKC Infographic

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In this infographic, you will see the following information for IGKC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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