Product Info Summary
| SKU: | PB9256 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KCND1 Antibody Picoband®
SKU/Catalog Number
PB9256
PB0255 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KCND1 Antibody Picoband® catalog # PB9256. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCND1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9256)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KCND1 recombinant protein (Position: T442-L647). Human KCND1 shares 90% amino acid (aa) sequence identity with mouse KCND1.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9256 is reactive to KCND1 in Human, Mouse
Observed Molecular Weight
71 kDa
Calculated molecular weight
71.3 kDa
Background of KCND1
Potassium voltage-gated channel, Shal-related subfamily, member 1 (KCND1), also known as Kv4.1, is a human gene. It is mapped to Xp11.23. KCND1 encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. The diverse functions of Voltage-gated potassium (Kv) channels include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. In addition to that, KCND1 is expressed at moderate levels in all tissues analyzed, with lower levels in skeletal muscle.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9256 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: Mouse Brain Tissue, HELA Whole Cell, COLO320 Whole Cell, A549 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-KCND1 Picoband antibody, PB9256, Western blotting
All lanes: Anti KCND1 (PB9256) at 0.5ug/ml
WB: Recombinant Human KCND1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Click image to see more details
Anti-KCND1 Picoband antibody, PB9256, Western blotting
All lanes: Anti KCND1 (PB9256) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Predicted bind size: 71KD
Observed bind size: 71KD
Specific Publications For Anti-KCND1 Antibody Picoband® (PB9256)
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3 Customer Q&As for Anti-KCND1 Antibody Picoband®
Question
I was wanting to use your anti-KCND1 antibody for WB for human embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
You can see on the product datasheet, PB9256 anti-KCND1 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
We want to test anti-KCND1 antibody PB9256 on human embryonic kidney for research purposes, then I may be interested in using anti-KCND1 antibody PB9256 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The products we sell, including anti-KCND1 antibody PB9256, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-22
Question
We are currently using anti-KCND1 antibody PB9256 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
S. Parker
Verified customer
Asked: 2016-01-28
Answer
The anti-KCND1 antibody (PB9256) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-01-28


