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Product Info Summary
| SKU: | A02788-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KCNJ1 Antibody Picoband®
SKU/Catalog Number
A02788-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KCNJ1 Antibody Picoband® catalog # A02788-2. Tested in WB applications. This antibody reacts with Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KCNJ1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02788-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human KCNJ1, which shares 80% amino acid (aa) sequence identity with both mouse and rat KCNJ1.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02788-2 is reactive to KCNJ1 in Monkey, Mouse, Rat
Observed Molecular Weight
45 kDa
Calculated molecular weight
44.8 kDa
Background of KCNJ1
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. It is activated by internal ATP and probably plays an important role in potassium homeostasis. The encoded protein has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Mutations in this gene have been associated with antenatal Bartter syndrome, which is characterized by salt wasting, hypokalemic alkalosis, hypercalciuria, and low blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02788-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Monkey, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue, mouse kidney tissue, monkey kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of KCNJ1 using anti-KCNJ1 antibody (A02788-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse kidney tissue lysates,
Lane 4: monkey kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNJ1 antigen affinity purified polyclonal antibody (Catalog # A02788-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNJ1 at approximately 45KD. The expected band size for KCNJ1 is at 45KD.
Specific Publications For Anti-KCNJ1 Antibody Picoband® (A02788-2)
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