Anti-KCNK12 Antibody

Product Name

Anti-KCNK12 Antibody

View all KCNK12 Antibodies

SKU/Catalog Number

A14172-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-KCNK12 Antibody (Catalog # A14172-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

KCNK12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-KCNK12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14172-1)

Host

Rabbit

Contents

KCNK12 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

KCNK12 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human KCNK12. The immunogen is located within amino acids 320 - 370 of KCNK12.

Cross-reactivity

KCNK12 antibody is predicted to not cross-react with other KCNK protein family members.

Reactive Species

A14172-1 is reactive to KCNK12 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

46.9 kDa

Background of KCNK12

KCNK13 and KCNK12 (also known as THIK1 and 2) are the first two members of a novel two pore-forming P domains K+ channels subfamily. The pore loop domain, a highly conserved region common to all potassium channels, is involved in determining potassium ion selectivity. Members of this family are all characterized by four transmembrane domains and may function to help influence the resting membrane potential of cells. KCNK12 is expressed mainly in the brain and lung, but also observed in the kidneys, heart and skeletal muscle. KCNK12 is closely related to KCNK13 (58% identity at the amino acid level), but could not yet been functionally expressed in vitro and may require other proteins to become active.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14172-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

KCNK12 antibody can be used for detection of KCNK12 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information KCNK12

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