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Product Info Summary
| SKU: | A08840-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-KDM8 Antibody Picoband®
SKU/Catalog Number
A08840-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-KDM8 Antibody Picoband® catalog # A18099. Tested in WB, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-KDM8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08840-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human KDM8 recombinant protein (Position: D4-D406). Human KDM8 shares 78.1% and 78.9% amino acid (aa) sequence identity with mouse and rat KDM8, respectively.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08840-2 is reactive to KDM8 in Human
Observed Molecular Weight
55 kDa
Calculated molecular weight
47.3 kDa
Background of KDM8
Lysine demethylase 8 is a protein that in humans is encoded by the KDM8 gene. This gene likely encodes a histone lysine demethylase. Studies of a similar protein in mouse indicate a potential role for this protein as a tumor suppressor. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08840-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Western blot analysis of KDM8 using anti-KDM8 antibody (A08840-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM8 antigen affinity purified polyclonal antibody (Catalog # A08840-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM8 at approximately 55 kDa. The expected band size for KDM8 is at 47 kDa.
Specific Publications For Anti-KDM8 Antibody Picoband® (A08840-2)
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