Anti-Keratocan Antibody Picoband™

KERA antibody

Boster Bio Anti-Keratocan Antibody Picoband™ catalog # PB9653. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: PB9653
Size: 100 μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-Keratocan Antibody Picoband™

View all KERA Antibodies

SKU/Catalog Number

PB9653

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Keratocan Antibody Picoband™ catalog # PB9653. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Keratocan Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9653)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Keratocan, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9653 is reactive to KERA in Human, Mouse

Applications

PB9653 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

40.509kDa

Background of KERA

Keratocan (KTN), also known as keratan sulfate proteoglycan keratocan, is a protein that in humans is encoded by the KERA gene. It is mapped to 12q22. The protein encoded by this gene is a keratan sulfate proteoglycan that is involved in corneal transparency. Defects in this gene are a cause of autosomal recessive cornea plana 2 (CNA2). Keratan sulfate proteoglycans (KSPGs) are members of the small leucine-rich proteoglycan (SLRP) family. KSPGs, particularly keratocan, lumican and mimecan, are important to the transparency of the cornea.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For KERA (Source: Uniprot.org, NCBI)

Gene Name

KERA

Full Name

Keratocan

Weight

40.509kDa

Superfamily

small leucine-rich proteoglycan (SLRP) family

Alternative Names

CNA2; KERA; Keratocan; KTN; SLRR2B; SLRR2BKeratan sulfate proteoglycan keratocan KERA CNA2, KTN, SLRR2B keratocan keratocan|keratan sulfate proteoglycan keratocan

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KERA, check out the KERA Infographic

KERA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KERA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Keratocan Antibody Picoband™

Question

My question regarding product PB9653, anti-Keratocan antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9653 anti-Keratocan antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-03

Question

Does anti-Keratocan antibody PB9653 work on monkey WB with duodenum?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

Our lab technicians have not validated anti-Keratocan antibody PB9653 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Keratocan antibody PB9653 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey duodenum in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-25

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-Keratocan antibody PB9653. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-05

Question

Is there a BSA free version of anti-Keratocan antibody PB9653 available?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Keratocan antibody PB9653 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-25

Question

We are currently using anti-Keratocan antibody PB9653 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-01

Answer

The anti-Keratocan antibody (PB9653) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-01

Question

I see that the anti-Keratocan antibody PB9653 works with WB, what is the protocol used to produce the result images on the product page?

R. Baker

Verified customer

Asked: 2015-08-21

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-08-21

Order DetailsPrice
PB9653

100μg

$370
PB9653-10ug

10μg sample (liquid)

$99
PB9653-Biotin

100 μg Biotin conjugated

$570
PB9653-Cy3

100 μg Cy3 conjugated

$570
PB9653-Dylight488

100 μg Dylight488 conjugated

$570
PB9653-Dylight550

100 μg Dylight550 conjugated

$570
PB9653-Dylight594

100 μg Dylight594 conjugated

$570
PB9653-FITC

100 μg FITC conjugated

$570
PB9653-HRP

100 μg HRP conjugated

$570
PB9653-APC

100 μg APC conjugated

$670
PB9653-PE

100 μg PE conjugated

$670
PB9653-carrier-free

Carrier Free

$370

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PB9653
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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