Product Info Summary
SKU: | M02770-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-KHSRP Antibody Picoband™ (monoclonal, 4F10D2)
SKU/Catalog Number
M02770-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KHSRP Antibody Picoband™ (monoclonal, 4F10D2) catalog # M02770-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-KHSRP Antibody Picoband™ (monoclonal, 4F10D2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02770-3)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
4F10D2
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human KHSRP recombinant protein (Position: D116-R478).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M02770-3 is reactive to KHSRP in Human
Applications
M02770-3 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
82 kDa
Calculated molecular weight
73.115kDa
Background of KHSRP
Far upstream element-binding protein 2 is a protein that in humans is encoded by the KHSRP gene. The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of KHSRP using anti-KHSRP antibody (M02770-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KHSRP antigen affinity purified monoclonal antibody (Catalog # M02770-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KHSRP at approximately 82 kDa. The expected band size for KHSRP is at 73 kDa.
Click image to see more details
Figure 2. IF analysis of KHSRP using anti-KHSRP antibody (M02770-3).
KHSRP was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL mouse anti-KHSRP Antibody (M02770-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of U251 cells using anti-KHSRP antibody (M02770-3).
Overlay histogram showing U251 cells stained with M02770-3 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-KHSRP Antibody (M02770-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For KHSRP (Source: Uniprot.org, NCBI)
Gene Name
KHSRP
Full Name
Far upstream element-binding protein 2
Weight
73.115kDa
Superfamily
KHSRP family
Alternative Names
FBP2far upstream element-binding protein 2; FUBP2p75; FUSE binding protein 2; FUSE-binding protein 2; KH type-splicing regulatory protein; KH-type splicing regulatory protein; KSRPMGC99676 KHSRP FBP2, FUBP2, KSRP, p75 KH-type splicing regulatory protein far upstream element-binding protein 2|FUSE-binding protein 2|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KHSRP, check out the KHSRP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KHSRP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-KHSRP Antibody Picoband™ (monoclonal, 4F10D2) (M02770-3)
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