Product Info Summary
SKU: | A03788 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Kinetochore protein Nuf2 Nuf2 Antibody
SKU/Catalog Number
A03788
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Kinetochore protein Nuf2 Nuf2 Antibody catalog # A03788. Tested in WB,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kinetochore protein Nuf2 Nuf2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03788)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Mnk1 around the phosphorylation site of T385.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03788 is reactive to Nuf2 in Human
Applications
A03788 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
54.594kDa
Background of Nuf2
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Fukunaga R., EMBO J. 16:1921-1933(1997).
Knauf U., Mol. Cell. Biol. 21:5500-5511(2001).
O'Loghlen A., Exp. Cell Res. 299:343-355(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Flow Cytometry validation of NUF2 using Anti-Kinetochore protein Nuf2 Nuf2 Antibody (A03788).
Flow cytometric analysis of A431 cells with Nuf2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Click image to see more details
Figure 1. Western blotting validation for Anti-Kinetochore protein Nuf2 Nuf2 Antibody A03788
Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1Protein Target Info & Infographic
Gene/Protein Information For Nuf2 (Source: Uniprot.org, NCBI)
Gene Name
Nuf2
Full Name
Kinetochore protein Nuf2
Weight
54.594kDa
Superfamily
NUF2 family
Alternative Names
cancer/testis antigen 106; CDCA1hsNuf2; cell division cycle associated 1; Cell division cycle-associated protein 1; CT106; hNuf2; kinetochore protein Nuf2; NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae); NUF2RhNuf2R
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nuf2, check out the Nuf2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nuf2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kinetochore protein Nuf2 Nuf2 Antibody (A03788)
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No publications found for A03788
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