Anti-KLC1 Antibody Picoband™ (monoclonal, 4F2D7)

Product Name

Anti-KLC1 Antibody Picoband™ (monoclonal, 4F2D7)

View all KLC1 Antibodies

SKU/Catalog Number

M04116-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KLC1 Antibody Picoband™ (monoclonal, 4F2D7) catalog # M04116-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-KLC1 Antibody Picoband™ (monoclonal, 4F2D7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04116-1)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

4F2D7

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human KLC1 recombinant protein (Position: M1-D537).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M04116-1 is reactive to KLC1 in Human, Mouse, Rat

Applications

M04116-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

65.31kDa

Background of KLC1

Kinesin light chain 1 is a protein that in humans is encoded by the KLC1 gene. Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of KLC1 using anti-KLC1 antibody (M04116-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-KLC1 antigen affinity purified monoclonal antibody (Catalog # M04116-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for KLC1 at approximately 65 kDa. The expected band size for KLC1 is at 65 kDa.

Gene/Protein Information For KLC1 (Source: Uniprot.org, NCBI)

Alternative Names

hKLC1B; hKLC1G; hKLC1J; hKLC1S; kinesin 2 60/70kDa; kinesin 2; kinesin light chain 1; KLC 1; KLChKLC1N; KNS2AhKLC1P; KNS2hKLC1R; medulloblastoma antigen MU-MB-2.50; MGC15245 KLC1 KLC, KNS2, KNS2A kinesin light chain 1 kinesin light chain 1|kinesin 2 60/70kDa|medulloblastoma antigen MU-MB-2.50

For more info on KLC1, check out the KLC1 Infographic

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In this infographic, you will see the following information for KLC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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