Anti-KLF11/Fklf Antibody

Boster Bio Anti-KLF11/Fklf Antibody catalog # A04277. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A04277
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-KLF11/Fklf Antibody

See all KLF11 products

SKU/Catalog Number

A04277

Size

100μl

Form

Liquid

Description

Boster Bio Anti-KLF11/Fklf Antibody catalog # A04277. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-KLF11/Fklf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04277)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from Internal of human KLF11.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04277 is reactive to KLF11 in Human, Mouse

Applications

A04277 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000

Validation Images & Assay Conditions

Gene/Protein Information For KLF11 (Source: Uniprot.Org, NCBI)

Uniprot ID

O14901

Gene ID

8462

Gene Name

KLF11

Full Name

Krueppel-like factor 11

Weight

55.139kDa

Superfamily

Sp1 C2H2-type zinc-finger protein family

Alternative Names

FKLFFKLF1; Krueppel-like factor 11; Kruppel-like factor 11; MODY7TGFB-inducible early growth response protein 2; TIEG-2; TIEG2TGFB inducible early growth response 2; Tieg3; Transforming growth factor-beta-inducible early growth response protein 2 KLF11 FKLF, FKLF1, MODY7, TIEG2, Tieg3 Kruppel like factor 11 Krueppel-like factor 11|TGFB-inducible early growth response protein 2|TIEG-2|transforming growth factor-beta-inducible early growth response protein 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KLF11, check out the KLF11 Infographic

KLF11 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KLF11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04277

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13 Customer Q&As for Anti-KLF11/Fklf Antibody

Question

Will anti-KLF11/Fklf antibody A04277 work on monkey WB with pancreas?

Verified Customer

Verified customer

Asked: 2020-04-16

Answer

Our lab technicians have not tested anti-KLF11/Fklf antibody A04277 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-KLF11/Fklf antibody A04277 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey pancreas in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-16

Question

I see that the anti-KLF11/Fklf antibody A04277 works with WB, what is the protocol used to produce the result images on the product page?

S. Johnson

Verified customer

Asked: 2019-10-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-23

Question

Does anti-KLF11/Fklf antibody A04277 work for WB with pancreas?

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

According to the expression profile of pancreas, KLF11 is highly expressed in pancreas. So, it is likely that anti-KLF11/Fklf antibody A04277 will work for WB with pancreas.

Boster Scientific Support

Answered: 2019-08-21

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-KLF11/Fklf antibody A04277. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-08

Question

Is this A04277 anti-KLF11/Fklf antibody reactive to the isotypes of KLF11?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

The immunogen of A04277 anti-KLF11/Fklf antibody is Synthesized peptide derived from Internal of human KLF11. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-26

Question

Would A04277 anti-KLF11/Fklf antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-06-09

Answer

You can see on the product datasheet, A04277 anti-KLF11/Fklf antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-06-09

Question

We are currently using anti-KLF11/Fklf antibody A04277 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-05

Answer

The anti-KLF11/Fklf antibody (A04277) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-05

Question

I was wanting to use your anti-KLF11/Fklf antibody for WB for human pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human pancreas identification?

K. Krishna

Verified customer

Asked: 2017-01-02

Answer

As indicated on the product datasheet, A04277 anti-KLF11/Fklf antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-01-02

Question

We are interested in to test anti-KLF11/Fklf antibody A04277 on human pancreas for research purposes, then I may be interested in using anti-KLF11/Fklf antibody A04277 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

W. Singh

Verified customer

Asked: 2015-11-30

Answer

The products we sell, including anti-KLF11/Fklf antibody A04277, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-11-30

Question

Is there a BSA free version of anti-KLF11/Fklf antibody A04277 available?

M. Miller

Verified customer

Asked: 2013-08-13

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-KLF11/Fklf antibody A04277 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-08-13

Question

Is a blocking peptide available for product anti-KLF11/Fklf antibody (A04277)?

J. Banerjee

Verified customer

Asked: 2013-04-23

Answer

We do provide the blocking peptide for product anti-KLF11/Fklf antibody (A04277). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-04-23

Question

I have attached the WB image, lot number and protocol we used for pancreas using anti-KLF11/Fklf antibody A04277. Please let me know if you require anything else.

L. Carter

Verified customer

Asked: 2013-02-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-02-14

Question

My question regarding product A04277, anti-KLF11/Fklf antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

P. Jha

Verified customer

Asked: 2013-01-08

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04277 anti-KLF11/Fklf antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-01-08

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04277
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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