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Product Info Summary
| SKU: | A05848 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Krueppel-like factor 12 KLF12 Antibody
SKU/Catalog Number
A05848
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Krueppel-like factor 12 KLF12 Antibody catalog # A05848. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Krueppel-like factor 12 KLF12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05848)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human KLF12.
Reactive Species
A05848 is reactive to KLF12 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05848 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of rat muscle cells using KLF12 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
Specific Publications For Anti-Krueppel-like factor 12 KLF12 Antibody (A05848)
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Krueppel-like factor 12 KLF12 Antibody
Question
I have attached the WB image, lot number and protocol we used for hypothalamus using anti-KLF12 antibody A05848. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-28
Question
We are currently using anti-KLF12 antibody A05848 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
L. Patel
Verified customer
Asked: 2019-02-01
Answer
The anti-KLF12 antibody (A05848) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-01
Question
My question regarding product A05848, anti-KLF12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05848 anti-KLF12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-19
