Anti-Krueppel-like factor 3 KLF3 Antibody

Product Name

Anti-Krueppel-like factor 3 KLF3 Antibody

View all Klf3 Antibodies

SKU/Catalog Number

PA1888

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Krueppel-like factor 3 KLF3 Antibody catalog # PA1888. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Krueppel-like factor 3 KLF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1888)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human KLF3, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1888 is reactive to Klf3 in Human, Mouse, Rat

Applications

PA1888 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

18 kDa

Calculated molecular weight

38.561kDa

Background of Klf3

KLF3 (Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene. It is also known as BKLF (Basic kruppel-like factor). KLF3 is a member of the Krüppel-like factors family of transcription factors. KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein (CtBP). Suske et al. (2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3. Suske et al. (2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively. Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors. The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197. Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Click image to see more details

Anti-KLF3 antibody, PA1888, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: HELA Cell Lysate

Gene/Protein Information For Klf3 (Source: Uniprot.org, NCBI)

Alternative Names

Basic krueppel-like factor; basic Kruppel-like factor; BKLFbasic kruppel like factor; CACCC-box-binding protein BKLF; Krueppel-like factor 3; Kruppel-like factor 3 (basic); MGC48279; TEF-2 Klf3|9930027G08Rik, AL024007, BKL, Bklf, Tef-2|Kruppel-like factor 3 (basic)|Krueppel-like factor 3|CACCC-box-binding protein BKLF|basic krueppel-like factor

For more info on Klf3, check out the Klf3 Infographic

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In this infographic, you will see the following information for Klf3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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