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Product Info Summary
| SKU: | A12797 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-KLHL21 Monoclonal Antibody
SKU/Catalog Number
A12797
Size
100μl
Form
Liquid
Description
Boster Bio Anti-KLHL21 Monoclonal Antibody catalog # A12797. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KLHL21 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12797)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2H4
Isotype
IgG
Immunogen
Purified recombinant fragment of human KLHL21 expressed in E. Coli.
Reactive Species
A12797 is reactive to KLHL21 in Human
Calculated molecular weight
66.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12797 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis using KLHL21 Monoclonal antibody against recombinant KLHL21 protein (1).
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain tissues with DAB staining using KLHL21 Monoclonal antibody.
Specific Publications For Anti-KLHL21 Monoclonal Antibody (A12797)
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4 Customer Q&As for Anti-KLHL21 Monoclonal Antibody
Question
My question regarding product A12797, anti-KLHL21 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12797 anti-KLHL21 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-30
Question
Is this A12797 anti-KLHL21 Monoclonal antibody reactive to the isotypes of KLHL21?
K. Anderson
Verified customer
Asked: 2020-04-20
Answer
The immunogen of A12797 anti-KLHL21 Monoclonal antibody is Purified recombinant fragment of human KLHL21 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-20
Question
We are currently using anti-KLHL21 Monoclonal antibody A12797 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
The anti-KLHL21 Monoclonal antibody (A12797) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-05
Question
Do you have a BSA free version of anti-KLHL21 Monoclonal antibody A12797 available?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-KLHL21 Monoclonal antibody A12797 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-29
